LOCUS       BBa_K607025             2459 bp    DNA     linear       05-Nov-2024
DEFINITION  PlasB_LasR-LVA_antipBad/araC with 0.6RBS
ACCESSION   BBa_K607025
VERSION     BBa_K607025.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     RBS             303..317
                     /label=RBS-1\Strong
     RBS             310..314
                     /label=RBS
     misc_feature    303..317
                     /label=BBa_B0030
     RBS             303..317
                     /label=BBa_B0030
     CDS             324..1040
                     /label=lasR
     misc_feature    1041..1073
                     /label=LVA
     misc_feature    803..803
                     /label=G
     misc_feature    1080..1104
                     /label=Help:Barcodes
     CDS             324..1104
                     /label=BBa_C0079
     promoter        1113..2322
                     /label=BBa_K607040
     stem_loop       2342..2385
                     /label=stem_loop
     misc_feature    2331..2410
                     /label=BBa_B0010
     terminator      2331..2410
                     /label=BBa_B0010
     stem_loop       2426..2445
                     /label=T7 TE
     misc_feature    2452..2452
                     /label=stop
     polyA_site      2446..2459
                     /label=polya
     misc_feature    2419..2459
                     /label=BBa_B0012
     terminator      2419..2459
                     /label=BBa_B0012
     terminator      2331..2459
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agattaaaga ggagaaatac tagatggcct tggttgacgg ttttcttgag ctggaacgct
      361 caagtggaaa attggagtgg agcgccatcc tccagaagat ggcgagcgac cttggattct
      421 cgaagatcct gttcggcctg ttgcctaagg acagccagga ctacgagaac gccttcatcg
      481 tcggcaacta cccggccgcc tggcgcgagc attacgaccg ggctggctac gcgcgggtcg
      541 acccgacggt cagtcactgt acccagagcg tactgccgat tttctgggaa ccgtccatct
      601 accagacgcg aaagcagcac gagttcttcg aggaagcctc ggccgccggc ctggtgtatg
      661 ggctgaccat gccgctgcat ggtgctcgcg gcgaactcgg cgcgctgagc ctcagcgtgg
      721 aagcggaaaa ccgggccgag gccaaccgtt tcatagagtc ggtcctgccg accctgtgga
      781 tgctcaagga ctacgcactg caaagcggtg ccggactggc cttcgaacat ccggtcagca
      841 aaccggtggt tctgaccagc cgggagaagg aagtgttgca gtggtgcgcc atcggcaaga
      901 ccagttggga gatatcggtt atctgcaact gctcggaagc caatgtgaac ttccatatgg
      961 gaaatattcg gcggaagttc ggtgtgacct cccgccgcgt agcggccatt atggccgtta
     1021 atttgggtct tattactctc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1081 actgatagtg ctagtgtaga tcactactag aggctagccc aaaaaaacgg gtatggagaa
     1141 acagtagaga gttgcgataa aaagcgtcag gtaggatccg ctaatcttat ggataaaaat
     1201 gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt tctgccgtga
     1261 ttatagacac ttttgttacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg
     1321 cttttaataa gcggggttac cggtttggtt agcgagaaga gccagtaaaa gacgcagtga
     1381 cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg gagtatgaaa
     1441 agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa tgcccatctg
     1501 gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat cgaccgaccg
     1561 ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt ggtgaaaaat
     1621 cagggacgag aatttgtttg ccgaccgggt gatattttgc tgttcccgcc aggagagatt
     1681 catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt ttactttcgt
     1741 ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa tacggggttc
     1801 tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaac
     1861 gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct tgagcaattg
     1921 ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga taatcgggta
     1981 cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga tatcgccagc
     2041 gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta
     2101 gggattagcg tcttaagctg gcgcgaggac caacgtatca gccaggcgaa gctgcttttg
     2161 agcaccaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc
     2221 tatttctcgc gggtatttaa aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt
     2281 tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat aatactagag ccaggcatca
     2341 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
     2401 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//