LOCUS BBa_K607026 2456 bp DNA linear 14-Nov-2024 DEFINITION PlasB_LasR-LVA_antipBad/araC with 1.0RBS ACCESSION BBa_K607026 VERSION BBa_K607026.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 misc_feature 183..200 /label=OP2 misc_feature 243..262 /label=OP1 promoter 254..259 /label=-35 promoter 277..282 /label=-10 promoter 138..294 /label=BBa_R0079 misc_feature 307..310 /label=conserved RBS 303..314 /label=BBa_B0034 CDS 321..1037 /label=lasR misc_feature 1038..1070 /label=LVA misc_feature 800..800 /label=G misc_feature 1077..1101 /label=Help:Barcodes CDS 321..1101 /label=BBa_C0079 promoter 1110..2319 /label=BBa_K607040 stem_loop 2339..2382 /label=stem_loop misc_feature 2328..2407 /label=BBa_B0010 terminator 2328..2407 /label=BBa_B0010 stem_loop 2423..2442 /label=T7 TE misc_feature 2449..2449 /label=stop polyA_site 2443..2456 /label=polya misc_feature 2416..2456 /label=BBa_B0012 terminator 2416..2456 /label=BBa_B0012 terminator 2328..2456 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg 181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg 241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag 301 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa 361 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga 421 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg 481 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc 541 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc 601 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc 661 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag 721 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc 781 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac 841 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca 901 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa 961 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt 1021 tgggtcttat tactctcgct gcaaacgacg aaaactacgc tttagtagct taataacact 1081 gatagtgcta gtgtagatca ctactagagg ctagcccaaa aaaacgggta tggagaaaca 1141 gtagagagtt gcgataaaaa gcgtcaggta ggatccgcta atcttatgga taaaaatgct 1201 atggcatagc aaagtgtgac gccgtgcaaa taatcaatgt ggacttttct gccgtgatta 1261 tagacacttt tgttacgcgt ttttgtcatg gctttggtcc cgctttgtta cagaatgctt 1321 ttaataagcg gggttaccgg tttggttagc gagaagagcc agtaaaagac gcagtgacgg 1381 caatgtctga tgcaatatgg acaattggtt tcttctctga atggcgggag tatgaaaagt 1441 atggctgaag cgcaaaatga tcccctgctg ccgggatact cgtttaatgc ccatctggtg 1501 gcgggtttaa cgccgattga ggccaacggt tatctcgatt tttttatcga ccgaccgctg 1561 ggaatgaaag gttatattct caatctcacc attcgcggtc agggggtggt gaaaaatcag 1621 ggacgagaat ttgtttgccg accgggtgat attttgctgt tcccgccagg agagattcat 1681 cactacggtc gtcatccgga ggctcgcgaa tggtatcacc agtgggttta ctttcgtccg 1741 cgcgcctact ggcatgaatg gcttaactgg ccgtcaatat ttgccaatac ggggttcttt 1801 cgcccggatg aagcgcacca gccgcatttc agcgacctgt ttgggcaaat cattaacgcc 1861 gggcaagggg aagggcgcta ttcggagctg ctggcgataa atctgcttga gcaattgtta 1921 ctgcggcgca tggaagcgat taacgagtcg ctccatccac cgatggataa tcgggtacgc 1981 gaggcttgtc agtacatcag cgatcacctg gcagacagca attttgatat cgccagcgtc 2041 gcacagcatg tttgcttgtc gccgtcgcgt ctgtcacatc ttttccgcca gcagttaggg 2101 attagcgtct taagctggcg cgaggaccaa cgtatcagcc aggcgaagct gcttttgagc 2161 accacccgga tgcctatcgc caccgtcggt cgcaatgttg gttttgacga tcaactctat 2221 ttctcgcggg tatttaaaaa atgcaccggg gccagcccga gcgagttccg tgccggttgt 2281 gaagaaaaag tgaatgatgt agccgtcaag ttgtcataat actagagcca ggcatcaaat 2341 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 2401 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //