LOCUS       BBa_K607028             2440 bp    DNA     linear       05-Nov-2024
DEFINITION  PlasB_LasR-DAS_antipBad/araC with 0.3RBS
ACCESSION   BBa_K607028
VERSION     BBa_K607028.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     RBS             303..315
                     /label=RBS-3\Weak
     RBS             309..312
                     /label=RBS
     misc_feature    303..315
                     /label=BBa_B0032
     RBS             303..315
                     /label=BBa_B0032
     CDS             322..1044
                     /label=BBa_C0179
     misc_feature    1053..1085
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    1053..1085
                     /label=BBa_M0052
     promoter        1094..2303
                     /label=BBa_K607040
     stem_loop       2323..2366
                     /label=stem_loop
     misc_feature    2312..2391
                     /label=BBa_B0010
     terminator      2312..2391
                     /label=BBa_B0010
     stem_loop       2407..2426
                     /label=T7 TE
     misc_feature    2433..2433
                     /label=stop
     polyA_site      2427..2440
                     /label=polya
     misc_feature    2400..2440
                     /label=BBa_B0012
     terminator      2400..2440
                     /label=BBa_B0012
     terminator      2312..2440
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agtcacacag gaaagtacta gatggccttg gttgacggtt ttcttgagct ggaacgctca
      361 agtggaaaat tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg
      421 aagatcctgt tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc
      481 ggcaactacc cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac
      541 ccgacggtca gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac
      601 cagacgcgaa agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg
      661 ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa
      721 gcggaaaacc gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg
      781 ctcaaggact acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa
      841 ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc
      901 agttgggaga tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga
      961 aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat
     1021 ttgggtctta ttactctcta ataatactag aggctgctaa cgacgaaaac tacgctgacg
     1081 cttcttacta gaggctagcc caaaaaaacg ggtatggaga aacagtagag agttgcgata
     1141 aaaagcgtca ggtaggatcc gctaatctta tggataaaaa tgctatggca tagcaaagtg
     1201 tgacgccgtg caaataatca atgtggactt ttctgccgtg attatagaca cttttgttac
     1261 gcgtttttgt catggctttg gtcccgcttt gttacagaat gcttttaata agcggggtta
     1321 ccggtttggt tagcgagaag agccagtaaa agacgcagtg acggcaatgt ctgatgcaat
     1381 atggacaatt ggtttcttct ctgaatggcg ggagtatgaa aagtatggct gaagcgcaaa
     1441 atgatcccct gctgccggga tactcgttta atgcccatct ggtggcgggt ttaacgccga
     1501 ttgaggccaa cggttatctc gattttttta tcgaccgacc gctgggaatg aaaggttata
     1561 ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa tcagggacga gaatttgttt
     1621 gccgaccggg tgatattttg ctgttcccgc caggagagat tcatcactac ggtcgtcatc
     1681 cggaggctcg cgaatggtat caccagtggg tttactttcg tccgcgcgcc tactggcatg
     1741 aatggcttaa ctggccgtca atatttgcca atacggggtt ctttcgcccg gatgaagcgc
     1801 accagccgca tttcagcgac ctgtttgggc aaatcattaa cgccgggcaa ggggaagggc
     1861 gctattcgga gctgctggcg ataaatctgc ttgagcaatt gttactgcgg cgcatggaag
     1921 cgattaacga gtcgctccat ccaccgatgg ataatcgggt acgcgaggct tgtcagtaca
     1981 tcagcgatca cctggcagac agcaattttg atatcgccag cgtcgcacag catgtttgct
     2041 tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt agggattagc gtcttaagct
     2101 ggcgcgagga ccaacgtatc agccaggcga agctgctttt gagcaccacc cggatgccta
     2161 tcgccaccgt cggtcgcaat gttggttttg acgatcaact ctatttctcg cgggtattta
     2221 aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg ttgtgaagaa aaagtgaatg
     2281 atgtagccgt caagttgtca taatactaga gccaggcatc aaataaaacg aaaggctcag
     2341 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     2401 cacactggct caccttcggg tgggcctttc tgcgtttata
//