LOCUS       BBa_K607029             2442 bp    DNA     linear       25-Nov-2024
DEFINITION  PlasB_LasR-DAS_antipBad/araC with 0.6RBS
ACCESSION   BBa_K607029
VERSION     BBa_K607029.1  
FEATURES             Location/Qualifiers
     misc_feature    1..41
                     /label=B0012
     misc_feature    50..129
                     /label=B0010
     terminator      1..129
                     /label=BBa_B0025
     misc_feature    183..200
                     /label=OP2
     misc_feature    243..262
                     /label=OP1
     promoter        254..259
                     /label=-35
     promoter        277..282
                     /label=-10
     promoter        138..294
                     /label=BBa_R0079
     RBS             303..317
                     /label=RBS-1\Strong
     RBS             310..314
                     /label=RBS
     misc_feature    303..317
                     /label=BBa_B0030
     RBS             303..317
                     /label=BBa_B0030
     CDS             324..1046
                     /label=BBa_C0179
     misc_feature    1055..1087
                     /label=AANDENYADAS  SsrA degradation tag
     misc_feature    1055..1087
                     /label=BBa_M0052
     promoter        1096..2305
                     /label=BBa_K607040
     stem_loop       2325..2368
                     /label=stem_loop
     misc_feature    2314..2393
                     /label=BBa_B0010
     terminator      2314..2393
                     /label=BBa_B0010
     stem_loop       2409..2428
                     /label=T7 TE
     misc_feature    2435..2435
                     /label=stop
     polyA_site      2429..2442
                     /label=polya
     misc_feature    2402..2442
                     /label=BBa_B0012
     terminator      2402..2442
                     /label=BBa_B0012
     terminator      2314..2442
                     /label=BBa_B0015
ORIGIN
        1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
       61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg
      181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg
      241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag
      301 agattaaaga ggagaaatac tagatggcct tggttgacgg ttttcttgag ctggaacgct
      361 caagtggaaa attggagtgg agcgccatcc tccagaagat ggcgagcgac cttggattct
      421 cgaagatcct gttcggcctg ttgcctaagg acagccagga ctacgagaac gccttcatcg
      481 tcggcaacta cccggccgcc tggcgcgagc attacgaccg ggctggctac gcgcgggtcg
      541 acccgacggt cagtcactgt acccagagcg tactgccgat tttctgggaa ccgtccatct
      601 accagacgcg aaagcagcac gagttcttcg aggaagcctc ggccgccggc ctggtgtatg
      661 ggctgaccat gccgctgcat ggtgctcgcg gcgaactcgg cgcgctgagc ctcagcgtgg
      721 aagcggaaaa ccgggccgag gccaaccgtt tcatagagtc ggtcctgccg accctgtgga
      781 tgctcaagga ctacgcactg caaagcggtg ccggactggc cttcgaacat ccggtcagca
      841 aaccggtggt tctgaccagc cgggagaagg aagtgttgca gtggtgcgcc atcggcaaga
      901 ccagttggga gatatcggtt atctgcaact gctcggaagc caatgtgaac ttccatatgg
      961 gaaatattcg gcggaagttc ggtgtgacct cccgccgcgt agcggccatt atggccgtta
     1021 atttgggtct tattactctc taataatact agaggctgct aacgacgaaa actacgctga
     1081 cgcttcttac tagaggctag cccaaaaaaa cgggtatgga gaaacagtag agagttgcga
     1141 taaaaagcgt caggtaggat ccgctaatct tatggataaa aatgctatgg catagcaaag
     1201 tgtgacgccg tgcaaataat caatgtggac ttttctgccg tgattataga cacttttgtt
     1261 acgcgttttt gtcatggctt tggtcccgct ttgttacaga atgcttttaa taagcggggt
     1321 taccggtttg gttagcgaga agagccagta aaagacgcag tgacggcaat gtctgatgca
     1381 atatggacaa ttggtttctt ctctgaatgg cgggagtatg aaaagtatgg ctgaagcgca
     1441 aaatgatccc ctgctgccgg gatactcgtt taatgcccat ctggtggcgg gtttaacgcc
     1501 gattgaggcc aacggttatc tcgatttttt tatcgaccga ccgctgggaa tgaaaggtta
     1561 tattctcaat ctcaccattc gcggtcaggg ggtggtgaaa aatcagggac gagaatttgt
     1621 ttgccgaccg ggtgatattt tgctgttccc gccaggagag attcatcact acggtcgtca
     1681 tccggaggct cgcgaatggt atcaccagtg ggtttacttt cgtccgcgcg cctactggca
     1741 tgaatggctt aactggccgt caatatttgc caatacgggg ttctttcgcc cggatgaagc
     1801 gcaccagccg catttcagcg acctgtttgg gcaaatcatt aacgccgggc aaggggaagg
     1861 gcgctattcg gagctgctgg cgataaatct gcttgagcaa ttgttactgc ggcgcatgga
     1921 agcgattaac gagtcgctcc atccaccgat ggataatcgg gtacgcgagg cttgtcagta
     1981 catcagcgat cacctggcag acagcaattt tgatatcgcc agcgtcgcac agcatgtttg
     2041 cttgtcgccg tcgcgtctgt cacatctttt ccgccagcag ttagggatta gcgtcttaag
     2101 ctggcgcgag gaccaacgta tcagccaggc gaagctgctt ttgagcacca cccggatgcc
     2161 tatcgccacc gtcggtcgca atgttggttt tgacgatcaa ctctatttct cgcgggtatt
     2221 taaaaaatgc accggggcca gcccgagcga gttccgtgcc ggttgtgaag aaaaagtgaa
     2281 tgatgtagcc gtcaagttgt cataatacta gagccaggca tcaaataaaa cgaaaggctc
     2341 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga
     2401 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta
//