LOCUS BBa_K607031 2400 bp DNA linear 25-Nov-2024 DEFINITION PlasB_LasR_antipBad/araC with 0.07RBS ACCESSION BBa_K607031 VERSION BBa_K607031.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 misc_feature 183..200 /label=OP2 misc_feature 243..262 /label=OP1 promoter 254..259 /label=-35 promoter 277..282 /label=-10 promoter 138..294 /label=BBa_R0079 misc_feature 309..312 /label=conserved RBS 303..316 /label=BBa_B0031 CDS 323..1045 /label=BBa_C0179 promoter 1054..2263 /label=BBa_K607040 stem_loop 2283..2326 /label=stem_loop misc_feature 2272..2351 /label=BBa_B0010 terminator 2272..2351 /label=BBa_B0010 stem_loop 2367..2386 /label=T7 TE misc_feature 2393..2393 /label=stop polyA_site 2387..2400 /label=polya misc_feature 2360..2400 /label=BBa_B0012 terminator 2360..2400 /label=BBa_B0012 terminator 2272..2400 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg 181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg 241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag 301 agtcacacag gaaacctact agatggcctt ggttgacggt tttcttgagc tggaacgctc 361 aagtggaaaa ttggagtgga gcgccatcct ccagaagatg gcgagcgacc ttggattctc 421 gaagatcctg ttcggcctgt tgcctaagga cagccaggac tacgagaacg ccttcatcgt 481 cggcaactac ccggccgcct ggcgcgagca ttacgaccgg gctggctacg cgcgggtcga 541 cccgacggtc agtcactgta cccagagcgt actgccgatt ttctgggaac cgtccatcta 601 ccagacgcga aagcagcacg agttcttcga ggaagcctcg gccgccggcc tggtgtatgg 661 gctgaccatg ccgctgcatg gtgctcgcgg cgaactcggc gcgctgagcc tcagcgtgga 721 agcggaaaac cgggccgagg ccaaccgttt catagagtcg gtcctgccga ccctgtggat 781 gctcaaggac tacgcactgc aaagcggtgc cggactggcc ttcgaacatc cggtcagcaa 841 accggtggtt ctgaccagcc gggagaagga agtgttgcag tggtgcgcca tcggcaagac 901 cagttgggag atatcggtta tctgcaactg ctcggaagcc aatgtgaact tccatatggg 961 aaatattcgg cggaagttcg gtgtgacctc ccgccgcgta gcggccatta tggccgttaa 1021 tttgggtctt attactctct aataatacta gaggctagcc caaaaaaacg ggtatggaga 1081 aacagtagag agttgcgata aaaagcgtca ggtaggatcc gctaatctta tggataaaaa 1141 tgctatggca tagcaaagtg tgacgccgtg caaataatca atgtggactt ttctgccgtg 1201 attatagaca cttttgttac gcgtttttgt catggctttg gtcccgcttt gttacagaat 1261 gcttttaata agcggggtta ccggtttggt tagcgagaag agccagtaaa agacgcagtg 1321 acggcaatgt ctgatgcaat atggacaatt ggtttcttct ctgaatggcg ggagtatgaa 1381 aagtatggct gaagcgcaaa atgatcccct gctgccggga tactcgttta atgcccatct 1441 ggtggcgggt ttaacgccga ttgaggccaa cggttatctc gattttttta tcgaccgacc 1501 gctgggaatg aaaggttata ttctcaatct caccattcgc ggtcaggggg tggtgaaaaa 1561 tcagggacga gaatttgttt gccgaccggg tgatattttg ctgttcccgc caggagagat 1621 tcatcactac ggtcgtcatc cggaggctcg cgaatggtat caccagtggg tttactttcg 1681 tccgcgcgcc tactggcatg aatggcttaa ctggccgtca atatttgcca atacggggtt 1741 ctttcgcccg gatgaagcgc accagccgca tttcagcgac ctgtttgggc aaatcattaa 1801 cgccgggcaa ggggaagggc gctattcgga gctgctggcg ataaatctgc ttgagcaatt 1861 gttactgcgg cgcatggaag cgattaacga gtcgctccat ccaccgatgg ataatcgggt 1921 acgcgaggct tgtcagtaca tcagcgatca cctggcagac agcaattttg atatcgccag 1981 cgtcgcacag catgtttgct tgtcgccgtc gcgtctgtca catcttttcc gccagcagtt 2041 agggattagc gtcttaagct ggcgcgagga ccaacgtatc agccaggcga agctgctttt 2101 gagcaccacc cggatgccta tcgccaccgt cggtcgcaat gttggttttg acgatcaact 2161 ctatttctcg cgggtattta aaaaatgcac cggggccagc ccgagcgagt tccgtgccgg 2221 ttgtgaagaa aaagtgaatg atgtagccgt caagttgtca taatactaga gccaggcatc 2281 aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg 2341 tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata //