LOCUS BBa_K607032 2399 bp DNA linear 23-Dec-2024 DEFINITION PlasB_LasR_antipBad/araC with 0.3RBS ACCESSION BBa_K607032 VERSION BBa_K607032.1 FEATURES Location/Qualifiers misc_feature 1..41 /label=B0012 misc_feature 50..129 /label=B0010 terminator 1..129 /label=BBa_B0025 misc_feature 183..200 /label=OP2 misc_feature 243..262 /label=OP1 promoter 254..259 /label=-35 promoter 277..282 /label=-10 promoter 138..294 /label=BBa_R0079 RBS 303..315 /label=RBS-3\Weak RBS 309..312 /label=RBS misc_feature 303..315 /label=BBa_B0032 RBS 303..315 /label=BBa_B0032 CDS 322..1044 /label=BBa_C0179 promoter 1053..2262 /label=BBa_K607040 stem_loop 2282..2325 /label=stem_loop misc_feature 2271..2350 /label=BBa_B0010 terminator 2271..2350 /label=BBa_B0010 stem_loop 2366..2385 /label=T7 TE misc_feature 2392..2392 /label=stop polyA_site 2386..2399 /label=polya misc_feature 2359..2399 /label=BBa_B0012 terminator 2359..2399 /label=BBa_B0012 terminator 2271..2399 /label=BBa_B0015 ORIGIN 1 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca 61 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt 121 gatgcctggt actagaggcc cctcgctgag cgcgtcccgg agctgggggc aacctagctg 181 ccacctgctt ttctgctagc tattccagcg aaaacataca gatttccggc gaaatcaagg 241 ctacctgcca gttctggcag gtttggccgc gggttctttt tggtacacga aagctactag 301 agtcacacag gaaagtacta gatggccttg gttgacggtt ttcttgagct ggaacgctca 361 agtggaaaat tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg 421 aagatcctgt tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc 481 ggcaactacc cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac 541 ccgacggtca gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac 601 cagacgcgaa agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg 661 ctgaccatgc cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa 721 gcggaaaacc gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg 781 ctcaaggact acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa 841 ccggtggttc tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc 901 agttgggaga tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga 961 aatattcggc ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat 1021 ttgggtctta ttactctcta ataatactag aggctagccc aaaaaaacgg gtatggagaa 1081 acagtagaga gttgcgataa aaagcgtcag gtaggatccg ctaatcttat ggataaaaat 1141 gctatggcat agcaaagtgt gacgccgtgc aaataatcaa tgtggacttt tctgccgtga 1201 ttatagacac ttttgttacg cgtttttgtc atggctttgg tcccgctttg ttacagaatg 1261 cttttaataa gcggggttac cggtttggtt agcgagaaga gccagtaaaa gacgcagtga 1321 cggcaatgtc tgatgcaata tggacaattg gtttcttctc tgaatggcgg gagtatgaaa 1381 agtatggctg aagcgcaaaa tgatcccctg ctgccgggat actcgtttaa tgcccatctg 1441 gtggcgggtt taacgccgat tgaggccaac ggttatctcg atttttttat cgaccgaccg 1501 ctgggaatga aaggttatat tctcaatctc accattcgcg gtcagggggt ggtgaaaaat 1561 cagggacgag aatttgtttg ccgaccgggt gatattttgc tgttcccgcc aggagagatt 1621 catcactacg gtcgtcatcc ggaggctcgc gaatggtatc accagtgggt ttactttcgt 1681 ccgcgcgcct actggcatga atggcttaac tggccgtcaa tatttgccaa tacggggttc 1741 tttcgcccgg atgaagcgca ccagccgcat ttcagcgacc tgtttgggca aatcattaac 1801 gccgggcaag gggaagggcg ctattcggag ctgctggcga taaatctgct tgagcaattg 1861 ttactgcggc gcatggaagc gattaacgag tcgctccatc caccgatgga taatcgggta 1921 cgcgaggctt gtcagtacat cagcgatcac ctggcagaca gcaattttga tatcgccagc 1981 gtcgcacagc atgtttgctt gtcgccgtcg cgtctgtcac atcttttccg ccagcagtta 2041 gggattagcg tcttaagctg gcgcgaggac caacgtatca gccaggcgaa gctgcttttg 2101 agcaccaccc ggatgcctat cgccaccgtc ggtcgcaatg ttggttttga cgatcaactc 2161 tatttctcgc gggtatttaa aaaatgcacc ggggccagcc cgagcgagtt ccgtgccggt 2221 tgtgaagaaa aagtgaatga tgtagccgtc aagttgtcat aatactagag ccaggcatca 2281 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt 2341 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata //