LOCUS BBa_K235020 2148 bp DNA linear 22-Dec-2024 DEFINITION [K235017][K235003] (pAra-controlled lambda repressor generator) ACCESSION BBa_K235020 VERSION BBa_K235020.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 misc_feature 1..1230 /label=BBa_K235017 CDS 1240..1947 /label=cI lambda misc_feature 1948..1980 /label=LVA misc_feature 1987..2011 /label=Help:Barcodes CDS 1237..2011 /label=BBa_C0051 stem_loop 2031..2074 /label=stem_loop misc_feature 2020..2099 /label=BBa_B0010 terminator 2020..2099 /label=BBa_B0010 stem_loop 2115..2134 /label=T7 TE misc_feature 2141..2141 /label=stop polyA_site 2135..2148 /label=polya misc_feature 2108..2148 /label=BBa_B0012 terminator 2108..2148 /label=BBa_B0012 terminator 2020..2148 /label=BBa_B0015 misc_feature 1237..2148 /label=BBa_K235003 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 1261 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 1321 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 1381 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 1441 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 1501 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 1561 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 1621 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 1681 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 1741 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 1801 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 1861 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 1921 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1981 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata // LOCUS BBa_K607037 2148 bp DNA linear 22-Dec-2024 DEFINITION pBAD/AraC generating cI transcription factor ACCESSION BBa_K607037 VERSION BBa_K607037.1 FEATURES Location/Qualifiers promoter 1080..1210 /label=Pbad promoter 1043..1114 /label=PC misc_feature 1..1079 /label=AraC promoter 1..1210 /label=BBa_I0500 misc_feature 1223..1226 /label=conserved RBS 1219..1230 /label=BBa_B0034 CDS 1240..1947 /label=cI lambda misc_feature 1948..1980 /label=LVA misc_feature 1987..2011 /label=Help:Barcodes CDS 1237..1986 /label=BBa_C0051 stem_loop 2031..2074 /label=stem_loop misc_feature 2020..2099 /label=BBa_B0010 terminator 2020..2099 /label=BBa_B0010 stem_loop 2115..2134 /label=T7 TE misc_feature 2141..2141 /label=stop polyA_site 2135..2148 /label=polya misc_feature 2108..2148 /label=BBa_B0012 terminator 2108..2148 /label=BBa_B0012 terminator 2020..2148 /label=BBa_B0015 ORIGIN 1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct 61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc 121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg 181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag 241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt 301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg 361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt 421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa 481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa 541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg 601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa 661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg 721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa 781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga 841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt 901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg 961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg 1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca 1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt 1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt 1201 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 1261 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 1321 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 1381 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 1441 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 1501 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 1561 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 1621 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 1681 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 1741 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 1801 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 1861 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 1921 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 1981 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata //