LOCUS       BBa_K607039             1279 bp    DNA     linear       17-May-2024
DEFINITION  hybB  cold shock promoter generating GFP
ACCESSION   BBa_K607039
VERSION     BBa_K607039.1  
FEATURES             Location/Qualifiers
     promoter        1..393
                     /label=BBa_J45503
     RBS             402..416
                     /label=RBS-1\Strong
     RBS             409..413
                     /label=RBS
     misc_feature    402..416
                     /label=BBa_B0030
     RBS             402..416
                     /label=BBa_B0030
     CDS             423..1142
                     /label=GFP protein
     CDS             423..1142
                     /label=BBa_E0040
     stem_loop       1162..1205
                     /label=stem_loop
     misc_feature    1151..1230
                     /label=BBa_B0010
     terminator      1151..1230
                     /label=BBa_B0010
     stem_loop       1246..1265
                     /label=T7 TE
     misc_feature    1272..1272
                     /label=stop
     polyA_site      1266..1279
                     /label=polya
     misc_feature    1239..1279
                     /label=BBa_B0012
     terminator      1239..1279
                     /label=BBa_B0012
     misc_feature    402..1279
                     /label=BBa_E0840
ORIGIN
        1 cgccgctatg gactggataa agagatggta atggatttct ttcgtgagaa taattcctgt
       61 tctacgttgc gcttttttat ggccggttat cgcctcgaaa attgatcaaa catacgtatt
      121 atcttgcttt aattaattac actaatgctt cttcccttcg ttttagcgcc ccgccgcagt
      181 atcatgatat cgataaccat aataaatgtg tggtaaatgg cgcatcgatc gcattattga
      241 ttttgcgatt gaggcaaaat atatgccagg tcttcgcaac ggaataacta taaatgactg
      301 gagataacac cctcatccat tctcacggca ttaaccgtcg tgatttcatg aagctttgtg
      361 cagcattagc cgccaccatg gggttaagta gcatactaga gattaaagag gagaaatact
      421 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
      481 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
      541 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
      601 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
      661 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
      721 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
      781 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
      841 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
      901 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
      961 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     1021 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     1081 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     1141 aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     1201 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     1261 gggcctttct gcgtttata
//