BBa_K608101
1
BBa_K608101
CcaR, green light response regulator
2011-09-13T11:00:00Z
2015-05-08T01:12:52Z
It was PCR-amplified from the genomic sequence of Synechocystis sp. PCC 6803.
The following primers were used:
XbaI_RBS_ccaR_up, TATTCTAGATTCACACAGGAAACCTCACTAATGAGAATTCTTTTAGTGGAGGATG
ccaR_SpeI_PstI_dw,GCCGGACTGCAGCGGCCGCTACTAGTCTAGTTTTTCCCTTGGCACAAAG
CcaR is the response regulator to the green light receptor CcaS.
It belongs to the family of OmpR regulator class. CcaR consists of an N-terminal receiver domain that can be phosphorylated by CcaS and a C-terminal DNA-binding domain that binds directly to the promoter region of cpcG2.
false
false
_780_
0
9115
9
It's complicated
false
We deleted an EcoRI restriction site in CcaR, in order to make it compatible to the iGEM standart.
false
Julia M??ller
BBa_K608101_sequence
1
atgatttgccgctggcggaaacccttgctgaagcattgagtgaccagctttacaccgttgatattgccaccgacgcttccctcgcctgggactatgcctcccgactggaatatgacctcgttattttggatgtgatgctgccggagttggacgggattaccctctgtcaaaaatggcgatcgcacagttatttaatgccaattttgatgatgacagccagggatacgatcaatgataaaatcacgggcttggatgcgggggcggatgattatgtggtcaagccagtggatttgggggagttatttgccagggtgcgagctttgttgcgtcggggttgtgcaacgtgccaaccagttttagagtgggggccaatcaggttggatccaagcacctatgaagttagttatgacaatgaggttttgtctttgacccgcaaggaatacagcattctggaattactactccgcaatggccgtcgggtgctaagtcggagcatgattatcgatagtatctggaagttggagagtcccccagaggaagatacggttaaggtgcatgtgcggagtttgcgacaaaaattaaaaagtgccggtttatcagcagatgccattgaaacggtccatggcattgggtatcgtctggccaatttaacggaaaaatctttgtgccaagggaaaaactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z