LOCUS BBa_K608351 948 bp DNA linear 05-Nov-2024 DEFINITION (correct) temperature sensitive promoter ACCESSION BBa_K608351 VERSION BBa_K608351.1 FEATURES Location/Qualifiers misc_feature 1..35 /label=BBa_J23114 misc_feature 48..51 /label=conserved misc_feature 44..55 /label=BBa_B0034 CDS 62..775 /label=cIts misc_feature 62..775 /label=BBa_K098997 misc_feature 797..837 /label=BBa_B0012 stem_loop 804..823 /label=T7 TE polyA_site 824..837 /label=polya misc_feature 830..830 /label=stop misc_feature 846..891 /label=BBa_B0011 stem_loop 858..880 /label=stem_loop misc_feature 900..916 /label=OR2 misc_feature 900..948 /label=BBa_R0051 promoter 914..919 /label=-35 misc_feature 924..940 /label=OR1 promoter 937..942 /label=-10 misc_feature 776..788 /label=conserved ORIGIN 1 tttatggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgagcaca aaaaagaaac cattaacaca agagcagctt gaggacgcac gtcgccttaa 121 agcaatttat gaaaaaaaga aaaatgaact tggcttatcc caggaatctg tcgcagacaa 181 gatggggatg gggcagtcag gcgttggtgc tttatttaat ggcatcaatg cattaaatgc 241 ttataacgcc gcattgcttg caaaaattct caaagttagc gttgaagaat ttagcccttc 301 aatcgccaga gaaatctacg agatgtatga agcggttagt atgcagccgt cacttagaag 361 tgagtatgag taccctgttt tttctcatgt tcaggcaggg atgttctcac ctgagcttag 421 aacctttacc aaaggtgatg cggagagatg ggtaagcaca accaaaaaag ccagtgattc 481 tgcattctgg cttgaggttg aaggtaattc catgaccgca ccaacaggct ccaagccaag 541 ctttcctgac ggaatgttaa ttctcgttga ccctgagcag gctgttgagc caggtgattt 601 ctgcatagcc agacttgggg gtgatgagtt taccttcaag aaactgatca gggatagcgg 661 tcaggtgttt ttacaaccac taaacccaca gtacccaatg atcccatgca atgagagttg 721 ttccgttgtg gggaaagtta tcgctagtca gtggcctgaa gagacgtttg gctgactcca 781 gcggccgcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac 841 tagagagaga atataaaaag ccagattatt aatccggctt ttttattatt ttactagagt 901 aacaccgtgc gtgttgacta ttttacctct ggcggtgata atggttgc //