BBa_K609000 1 BBa_K609000 nisI+pH induced promoter rcfB+ repressor LacR of lac operon 2011-09-30T11:00:00Z 2015-05-08T01:12:53Z The nisI gene comes from the Staphylococcus aureuis. The rcfB promoter is found in Lactococcus lactis IL1403, while the lacR gene is from Lactobacillus. delbrueckii subsp. lactis. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB, will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.And the nisI gene is a Nisin resistance gene which could be used in food. false false _781_ 0 8592 9 It's complicated false The part is designed to let Lactobacillus bulgaricus stop producing lactic acid when the external pH declines to 5.5 or lower. false Ke Liu component2148701 1 BBa_K609003 component2148704 1 BBa_K609001 annotation2148704 1 BBa_K609001 range2148704 1 878 2017 annotation2148701 1 BBa_K609003 range2148701 1 1 869 BBa_K609001 1 BBa_K609001 acid induced promoter rcfB + repressor lacR of lac operon 2011-09-30T11:00:00Z 2015-05-08T01:12:53Z The part is designed to let Lactobacillus bulgaricus stop producing lactic acid when the external pH declines to 5.5 or lower. When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB, will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria. false false _781_ 0 8592 9 Not in stock false The rcfB promoter is found in Lactococcus lactis IL1403, while the lacR gene is from Lactobacillus. delbrueckii subsp. lactis. false Ke Liu annotation2148327 1 lacR range2148327 1 113 1111 annotation2148328 1 rcfB range2148328 1 31 112 BBa_K609003 1 BBa_K609003 A Nisin resistance gene nisI for Lactobacillus bulgaricus 2011-09-30T11:00:00Z 2015-05-08T01:12:53Z The gene is found in Lactococcus lactis. This is a Nisin resistance gene nisI and the coding area has been optimized for a better function in Lactobacillus bulgaricus. false false _781_ 0 8592 9 Not in stock false For a reconstruction of food-grade plasmid. false Ke Liu annotation2148690 1 nisI range2148690 1 31 840 BBa_K609001_sequence 1 gtttcttcgaattcgcggccgcttctagagttttaatgatttacataaaaaatgatataatgaaatggctacagccttatataggcgaataaatgaacaaattagcgagtagatggctactatccgcgaagttgctaaggctgctggcgtttcaccagctactgtttcacgcgttctgaactacgaccaaactctgtcagttaacgaagctactcgccaaaagatcttcaagactgctgaagctatgcactaccacaagtcacgcaagactcgcaagtcaaagcaaaagcgcctggctatctgcctgtggtgcgaccaagaccaagaaatcaaggacctgtactactactcaatccgcacttcagctcaagctgaagctaagaagcaaggcctggaatcacaagttatctacccagctgacccactgccagacccagctgctctgtcaggcatcatcatgatcggctaccaacaatactcaccagaccgcctgaacgaagttaagaagtcaggcctgccactggttttcgttgacactgacactctgaagctgggctactgctcagttgttgctgacttcggccaagctatgcaagaagctctggaagttttctggggccaaggccgcgaacgcatcgctctgctggacggcgacctggactcaaacttcgacaagaacaacctggttgacttccgcttccgcgactacaagaagtcactggctgctcgcggccaatacgacccagacctggtttacgttggcaacttcactccacaatcaggctacgaagctatcaaggaagctctgaagtcaggctcattcccaaaggctctgatcgctgctaacgacgctatggctatcggcgctctgaaggctttcaaggaagctggcatcaaggttccagaagacgtttcactgatctcattcaacgacactactgctgctgagttcgctaacccagctctgacttcagttcacgttgaaactcaacaaatgggccgcgcttcagttaaggttatgaaggacctgctggacgacgacgaagctggcacttacaaggttactttcccaactaagctggtttaccgcgaatcatgcccaaaggcttaatactagtagcggccgctgcaggaagaaac BBa_K609000_sequence 1 gtttcttcgaattcgcggccgcttctagagatggataagaaaccgctctgctcgctcctgctggcggcggcctttattggccaggcccaaacggcccaggcgatgcgccgctacctgatcctgatcgttgctctgatcggcatcactggcctgtcaggctgctaccaaacttcacacaagaaggttcgcttcgacgaaggctcatacactaacttcatctacgacaacaagtcatacttcgttactgacaaggaaatcccacaagaaaacgttaacaactcaaaggttaagttctacaagctgctgatcgttgacatgaagtcagaaaagctgctgtcatcatcaaacaagaactcagttactctggttctgaacaacatctacgaagcttcagacaagtcactgtgcatgggcatcaacgaccgctactacaagatcctgccagaatcagacaagggcgctgttaaggctctgcgcctgcaaaacttcgacgttacttcagacatctcagacgacaacttcgttatcgacaagaacgactcacgcaagatcgactacatgggcaacatctactcaatctcagacactactgtttcagacgaagaactgggcgaataccaagacgttctggctgaagttcgcgttttcgactcagtttcaggcaagtcaatcccacgctcagaatggggccgcatcgacaaggacggctcaaactcaaagcaatcacgcactgaatgggactacggcgaaatccactcaatccgcggcaagtcactgactgaagctttcgctgttgaaatcaacgacgacttcaagctggctactaaggttggcaactaatactagtagcggccgctgcaggaagaaactactagaggtttcttcgaattcgcggccgcttctagagttttaatgatttacataaaaaatgatataatgaaatggctacagccttatataggcgaataaatgaacaaattagcgagtagatggctactatccgcgaagttgctaaggctgctggcgtttcaccagctactgtttcacgcgttctgaactacgaccaaactctgtcagttaacgaagctactcgccaaaagatcttcaagactgctgaagctatgcactaccacaagtcacgcaagactcgcaagtcaaagcaaaagcgcctggctatctgcctgtggtgcgaccaagaccaagaaatcaaggacctgtactactactcaatccgcacttcagctcaagctgaagctaagaagcaaggcctggaatcacaagttatctacccagctgacccactgccagacccagctgctctgtcaggcatcatcatgatcggctaccaacaatactcaccagaccgcctgaacgaagttaagaagtcaggcctgccactggttttcgttgacactgacactctgaagctgggctactgctcagttgttgctgacttcggccaagctatgcaagaagctctggaagttttctggggccaaggccgcgaacgcatcgctctgctggacggcgacctggactcaaacttcgacaagaacaacctggttgacttccgcttccgcgactacaagaagtcactggctgctcgcggccaatacgacccagacctggtttacgttggcaacttcactccacaatcaggctacgaagctatcaaggaagctctgaagtcaggctcattcccaaaggctctgatcgctgctaacgacgctatggctatcggcgctctgaaggctttcaaggaagctggcatcaaggttccagaagacgtttcactgatctcattcaacgacactactgctgctgagttcgctaacccagctctgacttcagttcacgttgaaactcaacaaatgggccgcgcttcagttaaggttatgaaggacctgctggacgacgacgaagctggcacttacaaggttactttcccaactaagctggtttaccgcgaatcatgcccaaaggcttaatactagtagcggccgctgcaggaagaaac BBa_K609003_sequence 1 gtttcttcgaattcgcggccgcttctagagatggataagaaaccgctctgctcgctcctgctggcggcggcctttattggccaggcccaaacggcccaggcgatgcgccgctacctgatcctgatcgttgctctgatcggcatcactggcctgtcaggctgctaccaaacttcacacaagaaggttcgcttcgacgaaggctcatacactaacttcatctacgacaacaagtcatacttcgttactgacaaggaaatcccacaagaaaacgttaacaactcaaaggttaagttctacaagctgctgatcgttgacatgaagtcagaaaagctgctgtcatcatcaaacaagaactcagttactctggttctgaacaacatctacgaagcttcagacaagtcactgtgcatgggcatcaacgaccgctactacaagatcctgccagaatcagacaagggcgctgttaaggctctgcgcctgcaaaacttcgacgttacttcagacatctcagacgacaacttcgttatcgacaagaacgactcacgcaagatcgactacatgggcaacatctactcaatctcagacactactgtttcagacgaagaactgggcgaataccaagacgttctggctgaagttcgcgttttcgactcagtttcaggcaagtcaatcccacgctcagaatggggccgcatcgacaaggacggctcaaactcaaagcaatcacgcactgaatgggactacggcgaaatccactcaatccgcggcaagtcactgactgaagctttcgctgttgaaatcaacgacgacttcaagctggctactaaggttggcaactaatactagtagcggccgctgcaggaagaaac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z