BBa_K609001
1
BBa_K609001
acid induced promoter rcfB + repressor lacR of lac operon
2011-09-30T11:00:00Z
2015-05-08T01:12:53Z
The part is designed to let Lactobacillus bulgaricus stop producing lactic acid when the external pH declines to 5.5 or lower.
When the external pH value declines to 5.5, rcfB promoter will be highly upregulated. Along with that, the lacR gene, which is downstream of rcfB, will be highly transcribed. The large translation of LacR, the repressor of lac operon in Lactobacillus delbrueckii, will inhibit the use of lactose in the bacteria.
false
false
_781_
0
8592
9
Not in stock
false
The rcfB promoter is found in Lactococcus lactis IL1403, while the lacR gene is from Lactobacillus. delbrueckii subsp. lactis.
false
Ke Liu
annotation2148328
1
rcfB
range2148328
1
31
112
annotation2148327
1
lacR
range2148327
1
113
1111
BBa_K609001_sequence
1
gtttcttcgaattcgcggccgcttctagagttttaatgatttacataaaaaatgatataatgaaatggctacagccttatataggcgaataaatgaacaaattagcgagtagatggctactatccgcgaagttgctaaggctgctggcgtttcaccagctactgtttcacgcgttctgaactacgaccaaactctgtcagttaacgaagctactcgccaaaagatcttcaagactgctgaagctatgcactaccacaagtcacgcaagactcgcaagtcaaagcaaaagcgcctggctatctgcctgtggtgcgaccaagaccaagaaatcaaggacctgtactactactcaatccgcacttcagctcaagctgaagctaagaagcaaggcctggaatcacaagttatctacccagctgacccactgccagacccagctgctctgtcaggcatcatcatgatcggctaccaacaatactcaccagaccgcctgaacgaagttaagaagtcaggcctgccactggttttcgttgacactgacactctgaagctgggctactgctcagttgttgctgacttcggccaagctatgcaagaagctctggaagttttctggggccaaggccgcgaacgcatcgctctgctggacggcgacctggactcaaacttcgacaagaacaacctggttgacttccgcttccgcgactacaagaagtcactggctgctcgcggccaatacgacccagacctggtttacgttggcaacttcactccacaatcaggctacgaagctatcaaggaagctctgaagtcaggctcattcccaaaggctctgatcgctgctaacgacgctatggctatcggcgctctgaaggctttcaaggaagctggcatcaaggttccagaagacgtttcactgatctcattcaacgacactactgctgctgagttcgctaacccagctctgacttcagttcacgttgaaactcaacaaatgggccgcgcttcagttaaggttatgaaggacctgctggacgacgacgaagctggcacttacaaggttactttcccaactaagctggtttaccgcgaatcatgcccaaaggcttaatactagtagcggccgctgcaggaagaaac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z