LOCUS       BBa_K611003             1725 bp    DNA     linear       29-May-2024
DEFINITION  E0240+I13453+B0034+C0040
ACCESSION   BBa_K611003
VERSION     BBa_K611003.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..739
                     /label=GFP protein
     CDS             20..739
                     /label=BBa_E0040
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    748..827
                     /label=BBa_B0010
     terminator      748..827
                     /label=BBa_B0010
     stem_loop       843..862
                     /label=T7 TE
     misc_feature    869..869
                     /label=stop
     polyA_site      863..876
                     /label=polya
     misc_feature    836..876
                     /label=BBa_B0012
     terminator      836..876
                     /label=BBa_B0012
     misc_feature    1..876
                     /label=BBa_E0240
     promoter        885..1014
                     /label=BBa_I13453
     misc_feature    1027..1030
                     /label=conserved
     RBS             1023..1034
                     /label=BBa_B0034
     CDS             1044..1660
                     /label=tetR
     misc_feature    1661..1694
                     /label=SsrA
     misc_feature    1701..1725
                     /label=Help:Barcodes
     CDS             1041..1700
                     /label=BBa_C0040
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca
      901 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
      961 acgcttttta tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag
     1021 agaaagagga gaaatactag atgtccagat tagataaaag taaagtgatt aacagcgcat
     1081 tagagctgct taatgaggtc ggaatcgaag gtttaacaac ccgtaaactc gcccagaagc
     1141 taggtgtaga gcagcctaca ttgtattggc atgtaaaaaa taagcgggct ttgctcgacg
     1201 ccttagccat tgagatgtta gataggcacc atactcactt ttgcccttta gaaggggaaa
     1261 gctggcaaga ttttttacgt aataacgcta aaagttttag atgtgcttta ctaagtcatc
     1321 gcgatggagc aaaagtacat ttaggtacac ggcctacaga aaaacagtat gaaactctcg
     1381 aaaatcaatt agccttttta tgccaacaag gtttttcact agagaatgca ttatatgcac
     1441 tcagcgctgt ggggcatttt actttaggtt gcgtattgga agatcaagag catcaagtcg
     1501 ctaaagaaga aagggaaaca cctactactg atagtatgcc gccattatta cgacaagcta
     1561 tcgaattatt tgatcaccaa ggtgcagagc cagccttctt attcggcctt gaattgatca
     1621 tatgcggatt agaaaaacaa cttaaatgtg aaagtgggtc cgctgcaaac gacgaaaact
     1681 acgctttagt agcttaataa cactgatagt gctagtgtag atcac
//