LOCUS       BBa_K611004             2193 bp    DNA     linear       10-Jun-2024
DEFINITION  E0240+I13453+B0034+C0012
ACCESSION   BBa_K611004
VERSION     BBa_K611004.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..739
                     /label=GFP protein
     CDS             20..739
                     /label=BBa_E0040
     stem_loop       759..802
                     /label=stem_loop
     misc_feature    748..827
                     /label=BBa_B0010
     terminator      748..827
                     /label=BBa_B0010
     stem_loop       843..862
                     /label=T7 TE
     misc_feature    869..869
                     /label=stop
     polyA_site      863..876
                     /label=polya
     misc_feature    836..876
                     /label=BBa_B0012
     terminator      836..876
                     /label=BBa_B0012
     misc_feature    1..876
                     /label=BBa_E0240
     promoter        885..1014
                     /label=BBa_I13453
     misc_feature    1027..1030
                     /label=conserved
     RBS             1023..1034
                     /label=BBa_B0034
     misc_feature    2130..2168
                     /label=LVA
     CDS             1041..2168
                     /label=lacI-LVA
     misc_feature    1041..2168
                     /label=BBa_C0012
     misc_feature    2169..2193
                     /label=Help:Barcodes
     CDS             1041..2168
                     /label=BBa_C0012
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      781 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
      841 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagacattg attatttgca
      901 cggcgtcaca ctttgctatg ccatagcatt tttatccata agattagcgg atcctacctg
      961 acgcttttta tcgcaactct ctactgtttc tccataccgt ttttttgggc tagctactag
     1021 agaaagagga gaaatactag atggtgaatg tgaaaccagt aacgttatac gatgtcgcag
     1081 agtatgccgg tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt
     1141 ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc
     1201 gcgtggcaca acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc
     1261 tggccctgca cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg
     1321 gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg
     1381 tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc
     1441 aggatgccat tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct
     1501 ctgaccagac acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg
     1561 tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt
     1621 ctgtctcggc gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc
     1681 agccgatagc ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc
     1741 aaatgctgaa tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc
     1801 tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag
     1861 tgggatacga cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac
     1921 aggattttcg cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc
     1981 aggcggtgaa gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg
     2041 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     2101 gacaggtttc ccgactggaa agcgggcagg ctgcaaacga cgaaaactac gctttagtag
     2161 cttaataact ctgatagtgc tagtgtagat ctc
//