BBa_K611008
1
BBa_K611008
R0040+B0034+E0040+B0015+I13453+B0034
2011-09-17T11:00:00Z
2015-05-08T01:12:53Z
Built from existing parts in the registry though BioBrick assembly.
Released HQ 2013
This part is used to characterize mutants of BBa_C0040 (TetR). By adding a mutant of BBa_C0040 after this part, one can measure several of its properties through its regulation of GFP expression. Concentration of the added TetR mutant can be varied through different arabinose concentrations as long as AraC is present.
true
false
_783_
0
10185
9
Discontinued
false
There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3).
false
Nicholas Csicsery
annotation2133178
1
BBa_R0040
range2133178
1
1
54
annotation2133182
1
-10
range2133182
1
43
48
annotation2133179
1
TetR 1
range2133179
1
1
19
annotation2133180
1
-35
range2133180
1
20
25
annotation2133181
1
TetR 2
range2133181
1
26
44
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986787
1
-10
range1986787
1
43
48
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986785
1
-35
range1986785
1
20
25
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K611008_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z