LOCUS       BBa_K611012             1724 bp    DNA     linear       24-Nov-2024
DEFINITION  pTet Characterization Construct
ACCESSION   BBa_K611012
VERSION     BBa_K611012.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     terminator      747..875
                     /label=BBa_B0015
     promoter        884..1013
                     /label=BBa_I13453
     misc_feature    1026..1029
                     /label=conserved
     RBS             1022..1033
                     /label=BBa_B0034
     CDS             1043..1659
                     /label=tetR
     misc_feature    1660..1693
                     /label=SsrA
     misc_feature    1700..1724
                     /label=Help:Barcodes
     CDS             1040..1724
                     /label=BBa_C0040
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacattga ttatttgcac
      901 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga
      961 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga
     1021 gaaagaggag aaatactaga tgtccagatt agataaaagt aaagtgatta acagcgcatt
     1081 agagctgctt aatgaggtcg gaatcgaagg tttaacaacc cgtaaactcg cccagaagct
     1141 aggtgtagag cagcctacat tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc
     1201 cttagccatt gagatgttag ataggcacca tactcacttt tgccctttag aaggggaaag
     1261 ctggcaagat tttttacgta ataacgctaa aagttttaga tgtgctttac taagtcatcg
     1321 cgatggagca aaagtacatt taggtacacg gcctacagaa aaacagtatg aaactctcga
     1381 aaatcaatta gcctttttat gccaacaagg tttttcacta gagaatgcat tatatgcact
     1441 cagcgctgtg gggcatttta ctttaggttg cgtattggaa gatcaagagc atcaagtcgc
     1501 taaagaagaa agggaaacac ctactactga tagtatgccg ccattattac gacaagctat
     1561 cgaattattt gatcaccaag gtgcagagcc agccttctta ttcggccttg aattgatcat
     1621 atgcggatta gaaaaacaac ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta
     1681 cgctttagta gcttaataac actgatagtg ctagtgtaga tcac
//