LOCUS       BBa_K611013             2192 bp    DNA     linear       22-Dec-2024
DEFINITION  pLacI Characterization Construct
ACCESSION   BBa_K611013
VERSION     BBa_K611013.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     terminator      747..875
                     /label=BBa_B0015
     promoter        884..1013
                     /label=BBa_I13453
     misc_feature    1026..1029
                     /label=conserved
     RBS             1022..1033
                     /label=BBa_B0034
     misc_feature    2129..2167
                     /label=LVA
     CDS             1040..2167
                     /label=lacI-LVA
     misc_feature    1040..2167
                     /label=BBa_C0012
     misc_feature    2168..2192
                     /label=Help:Barcodes
     CDS             1040..2167
                     /label=BBa_C0012
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacattga ttatttgcac
      901 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga
      961 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga
     1021 gaaagaggag aaatactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga
     1081 gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc
     1141 tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg
     1201 cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct
     1261 ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg
     1321 tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt
     1381 gcacaatctt ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca
     1441 ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc
     1501 tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt
     1561 ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc
     1621 tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca
     1681 gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca
     1741 aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct
     1801 gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt
     1861 gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca
     1921 ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca
     1981 ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc
     2041 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     2101 acaggtttcc cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc
     2161 ttaataactc tgatagtgct agtgtagatc tc
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