LOCUS       BBa_K611014             1814 bp    DNA     linear       22-Dec-2024
DEFINITION  cI Repressible Promoter Characterization Construct
ACCESSION   BBa_K611014
VERSION     BBa_K611014.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..738
                     /label=GFP protein
     CDS             19..738
                     /label=BBa_E0040
     stem_loop       758..801
                     /label=stem_loop
     misc_feature    747..826
                     /label=BBa_B0010
     terminator      747..826
                     /label=BBa_B0010
     stem_loop       842..861
                     /label=T7 TE
     misc_feature    868..868
                     /label=stop
     polyA_site      862..875
                     /label=polya
     misc_feature    835..875
                     /label=BBa_B0012
     terminator      835..875
                     /label=BBa_B0012
     terminator      747..875
                     /label=BBa_B0015
     promoter        884..1013
                     /label=BBa_I13453
     misc_feature    1026..1029
                     /label=conserved
     RBS             1022..1033
                     /label=BBa_B0034
     CDS             1043..1750
                     /label=cI lambda
     misc_feature    1751..1783
                     /label=LVA
     misc_feature    1790..1814
                     /label=Help:Barcodes
     CDS             1040..1814
                     /label=BBa_C0051
ORIGIN
        1 aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt
       61 cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
      121 ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
      181 gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac
      241 ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
      301 gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
      361 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
      421 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
      481 gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
      541 agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
      601 ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
      661 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
      721 gaactataca aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      781 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      841 tggctcacct tcgggtgggc ctttctgcgt ttatatacta gagacattga ttatttgcac
      901 ggcgtcacac tttgctatgc catagcattt ttatccataa gattagcgga tcctacctga
      961 cgctttttat cgcaactctc tactgtttct ccataccgtt tttttgggct agctactaga
     1021 gaaagaggag aaatactaga tgagcacaaa aaagaaacca ttaacacaag agcagcttga
     1081 ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg gcttatccca
     1141 ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt tatttaatgg
     1201 catcaatgca ttaaatgctt ataacgccgc attgcttgca aaaattctca aagttagcgt
     1261 tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag cggttagtat
     1321 gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc aggcagggat
     1381 gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg taagcacaac
     1441 caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca tgaccgcacc
     1501 aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc ctgagcaggc
     1561 tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta ccttcaagaa
     1621 actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt acccaatgat
     1681 cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt ggcctgaaga
     1741 gacgtttggc gctgcaaacg acgaaaacta cgctttagta gcttaataac gctgatagtg
     1801 ctagtgtaga tcgc
//