BBa_K611021
1
BBa_K611021
LacI Promoter Variant #1
2011-09-18T11:00:00Z
2015-05-08T01:12:53Z
Mutagenic PCR of BBa_R0010.
Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.
false
false
_783_
0
10185
9
Not in stock
false
We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.
false
Nicholas Csicsery
annotation2135634
1
a->t
range2135634
1
196
196
annotation2135629
1
-35
range2135629
1
137
142
annotation2135627
1
a->g
range2135627
1
103
103
annotation2135633
1
t->c
range2135633
1
176
176
annotation2135628
1
a->g
range2135628
1
108
108
annotation2135632
1
LacI binding site
range2135632
1
166
200
annotation2135625
1
a->g
range2135625
1
40
40
annotation2135635
1
start
range2135635
1
173
173
annotation2135624
1
t->a
range2135624
1
37
37
annotation2135636
1
end of LacI coding region (inactive)
range2135636
1
1
88
annotation2135626
1
CAP binding site
range2135626
1
89
126
annotation2135631
1
-10
range2135631
1
161
166
annotation2135630
1
t->c
range2135630
1
151
151
BBa_K611021_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgaatcgttaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattagtgtgggttagctcactcattaggcaccccaggctttacactttatgcctccggctcgtatgttgtgtggaatcgtgagcggataacaatttctcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z