BBa_K611027
1
BBa_K611027
LacI Promoter Variant #7
2011-09-18T11:00:00Z
2015-05-08T01:12:53Z
Mutagenic PCR of BBa_R0010.
Parts K611021 through K611027 are variants of the wild type LacI repressible promoter, BBa_R0010. These parts were generated through three rounds of mutagenic PCR and each have around 1 to 7 mutations. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs.
false
false
_783_
0
10185
9
Not in stock
false
We chose to mutate this part from a well known promoter so that any changes in behavior would be easy to observe.
false
Nicholas Csicsery
annotation2134968
1
LacI binding site
range2134968
1
166
200
annotation2136225
1
start
range2136225
1
173
173
annotation2134967
1
a->t
range2134967
1
188
188
annotation2136221
1
end of LacI coding region (inactive)
range2136221
1
1
88
annotation2136222
1
CAP binding site
range2136222
1
89
126
annotation2134966
1
t->a
range2134966
1
166
166
annotation2136224
1
-10
range2136224
1
161
166
annotation2134965
1
a->g
range2134965
1
44
44
annotation2136223
1
-35
range2136223
1
137
142
BBa_K611027_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattagtgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgtagtgtggaattgtgagcggatatcaatttcacaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z