LOCUS       BBa_K611035             2400 bp    DNA     linear       22-Nov-2024
DEFINITION  LacI Promoter Variant #5 in Characterization Construct
ACCESSION   BBa_K611035
VERSION     BBa_K611035.1  
FEATURES             Location/Qualifiers
     misc_feature    30..30
                     /label=t->c
     misc_feature    117..117
                     /label=a->c
     misc_feature    123..123
                     /label=t->c
     misc_feature    166..200
                     /label=LacI binding site
     CDS             1..88
                     /label=	end of LacI coding region (inactive)
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_K611025
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..946
                     /label=GFP protein
     CDS             227..946
                     /label=BBa_E0040
     stem_loop       966..1009
                     /label=stem_loop
     misc_feature    955..1034
                     /label=BBa_B0010
     terminator      955..1034
                     /label=BBa_B0010
     stem_loop       1050..1069
                     /label=T7 TE
     misc_feature    1076..1076
                     /label=stop
     polyA_site      1070..1083
                     /label=polya
     misc_feature    1043..1083
                     /label=BBa_B0012
     terminator      1043..1083
                     /label=BBa_B0012
     terminator      955..1083
                     /label=BBa_B0015
     promoter        1092..1221
                     /label=BBa_I13453
     misc_feature    1234..1237
                     /label=conserved
     RBS             1230..1241
                     /label=BBa_B0034
     misc_feature    2337..2375
                     /label=LVA
     CDS             1248..2375
                     /label=lacI-LVA
     misc_feature    1248..2375
                     /label=BBa_C0012
     misc_feature    2376..2400
                     /label=Help:Barcodes
     CDS             1248..2375
                     /label=BBa_C0012
     misc_feature    209..2400
                     /label=BBa_K611013
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtc ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctccctc
      121 atcaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagccaggc
      961 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
     1021 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     1081 atatactaga gacattgatt atttgcacgg cgtcacactt tgctatgcca tagcattttt
     1141 atccataaga ttagcggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
     1201 ataccgtttt tttgggctag ctactagaga aagaggagaa atactagatg gtgaatgtga
     1261 aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
     1321 gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     1381 tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     1441 tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     1501 cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     1561 gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     1621 tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     1681 atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     1741 cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     1801 tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     1861 ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     1921 ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
     1981 tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     2041 tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     2101 atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     2161 accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     2221 cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     2281 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcaggctg
     2341 caaacgacga aaactacgct ttagtagctt aataactctg atagtgctag tgtagatctc
//