LOCUS       BBa_K611059             1786 bp    DNA     linear       17-May-2024
DEFINITION  pTet Promoter Variant #9 in Characterization Construct
ACCESSION   BBa_K611059
VERSION     BBa_K611059.1  
FEATURES             Location/Qualifiers
     misc_feature    45..45
                     /label=t->c
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    26..42
                     /label=TetR 2
     promoter        20..25
                     /label=-35
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_K611049
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..800
                     /label=GFP protein
     CDS             81..800
                     /label=BBa_E0040
     stem_loop       820..863
                     /label=stem_loop
     misc_feature    809..888
                     /label=BBa_B0010
     terminator      809..888
                     /label=BBa_B0010
     stem_loop       904..923
                     /label=T7 TE
     misc_feature    930..930
                     /label=stop
     polyA_site      924..937
                     /label=polya
     misc_feature    897..937
                     /label=BBa_B0012
     terminator      897..937
                     /label=BBa_B0012
     terminator      809..937
                     /label=BBa_B0015
     promoter        946..1075
                     /label=BBa_I13453
     misc_feature    1088..1091
                     /label=conserved
     RBS             1084..1095
                     /label=BBa_B0034
     CDS             1105..1721
                     /label=tetR
     misc_feature    1722..1755
                     /label=SsrA
     misc_feature    1762..1786
                     /label=Help:Barcodes
     CDS             1102..1786
                     /label=BBa_C0040
     misc_feature    63..1786
                     /label=BBa_K611012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagacactga gcactactag
       61 agaaagagga gaaatactag atgcgtaaag gagaagaact tttcactgga gttgtcccaa
      121 ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg
      181 aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac
      241 ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc tttgcgagat
      301 acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa ggttatgtac
      361 aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt
      421 ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg
      481 gaaacattct tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
      541 cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac attgaagatg
      601 gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc
      661 ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat cccaacgaaa
      721 agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca catggcatgg
      781 atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
      841 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
      901 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagacatt gattatttgc
      961 acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg gatcctacct
     1021 gacgcttttt atcgcaactc tctactgttt ctccataccg tttttttggg ctagctacta
     1081 gagaaagagg agaaatacta gatgtccaga ttagataaaa gtaaagtgat taacagcgca
     1141 ttagagctgc ttaatgaggt cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag
     1201 ctaggtgtag agcagcctac attgtattgg catgtaaaaa ataagcgggc tttgctcgac
     1261 gccttagcca ttgagatgtt agataggcac catactcact tttgcccttt agaaggggaa
     1321 agctggcaag attttttacg taataacgct aaaagtttta gatgtgcttt actaagtcat
     1381 cgcgatggag caaaagtaca tttaggtaca cggcctacag aaaaacagta tgaaactctc
     1441 gaaaatcaat tagccttttt atgccaacaa ggtttttcac tagagaatgc attatatgca
     1501 ctcagcgctg tggggcattt tactttaggt tgcgtattgg aagatcaaga gcatcaagtc
     1561 gctaaagaag aaagggaaac acctactact gatagtatgc cgccattatt acgacaagct
     1621 atcgaattat ttgatcacca aggtgcagag ccagccttct tattcggcct tgaattgatc
     1681 atatgcggat tagaaaaaca acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac
     1741 tacgctttag tagcttaata acactgatag tgctagtgta gatcac
//