##gff-version 3
##sequence-region BBa_K611059 1 1786
BBa_K611059	.	promoter	1	54	.	+	0	ID=BBa_K611049;Name=BBa_K611049
BBa_K611059	.	sequence_alteration	45	45	.	+	0	ID=annotation2143817;Name=t->c;Parent=BBa_K611049
BBa_K611059	.	promoter	43	48	.	+	0	ID=annotation2143851;Name=-10;Parent=BBa_K611049
BBa_K611059	.	non_covalent_binding_site	26	42	.	+	0	ID=annotation2143849;Name=TetR 2;Parent=BBa_K611049
BBa_K611059	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation2143830;Name=TetR 1;Parent=BBa_K611049
BBa_K611059	.	promoter	20	25	.	+	0	ID=annotation2143850;Name=-35;Parent=BBa_K611049
BBa_K611059	.	engineered_region	63	1786	.	+	0	ID=BBa_K611012;Name=BBa_K611012
BBa_K611059	.	CDS	81	800	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K611012
BBa_K611059	.	CDS	81	800	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K611059	.	ribosome_entry_site	1084	1095	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K611012
BBa_K611059	.	conserved_region	1088	1091	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K611059	.	CDS	1102	1786	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_K611012
BBa_K611059	.	tag	1722	1755	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_K611059	.	engineered_tag	1762	1786	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_K611059	.	CDS	1105	1721	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_K611059	.	promoter	946	1075	.	+	0	ID=BBa_I13453;Name=BBa_I13453;Parent=BBa_K611012
BBa_K611059	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K611012
BBa_K611059	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K611059	.	terminator	809	937	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K611012
BBa_K611059	.	terminator	897	937	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K611059	.	engineered_region	897	937	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K611059	.	polyA_site	924	937	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K611059	.	stop_codon	930	930	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K611059	.	stem_loop	904	923	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K611059	.	terminator	809	888	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K611059	.	engineered_region	809	888	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K611059	.	stem_loop	820	863	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K611059
tccctatcagtgatagagattgacatccctatcagtgatagagacactgagcactactagagaaagaggagaaatacta
gatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaa
ttttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaac
tacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaa
acagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaac
tacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaag
aagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaa
gaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaa
aatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatc
ccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactata
caaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttg
tttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaca
ttgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctt
tttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtc
cagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgt
aaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgcct
tagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaa
taacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaa
aaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcac
tcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaac
acctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttc
ttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaa
actacgctttagtagcttaataacactgatagtgctagtgtagatcac