BBa_K611059
type: ComponentDefinition
persistent identity: BBa K611059
display id: BBa_K611059
version: 1
was generated by: iGEM to SBOL conversion
description: pTet Promoter Variant #9 in Characterization Construct
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:53Z
mutable provenance: Mutagenic PCR of the Tet repressible promoter, R0040, and standard BioBrick assembly of parts from the registry.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part contains a variant of the wild type Tet repressible promoter, and is followed by the pTet characterization construct, K611013. This allows for testing of this particular mutant promoter at various concentrations of repressor. The mutated pTet promoter in this plasmid expresses GFP and is followed by an arabinose inducible promoter, pBad, which expresses the TetR repressor. Thus, a user can increase the concentration of repressor by inducing pBad with arabinose. This plasmid is very useful for the further characterization of K611049.
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).
star: false
creator: Nicholas Csicsery
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K611012
component: BBa_K611049
sequence annotation: BBa_K611049
sequence annotation: BBa_K611012
sequence: https://synbiohub.org/public/igem/BBa_K611059_sequence/1

BBa_K611012
type: Component
persistent identity: component2273710
display id: component2273710
version: 1
top level: BBa_K611059
definition: BBa_K611012
access: public

BBa_K611049
type: Component
persistent identity: component2273691
display id: component2273691
version: 1
top level: BBa_K611059
definition: BBa_K611049
access: public

BBa_K611049
type: SequenceAnnotation
persistent identity: annotation2273691
display id: annotation2273691
version: 1
top level: BBa_K611059
location: https://synbiohub.org/public/igem/BBa_K611059/annotation2273691/range2273691/1
component: BBa_K611049

https://synbiohub.org/public/igem/BBa_K611059/annotation2273691/range2273691/1
type: Range
persistent identity: range2273691
display id: range2273691
version: 1
top level: BBa_K611059
direction: direction/reverse
start: 1
end: 54
orientation: inline

BBa_K611012
type: SequenceAnnotation
persistent identity: annotation2273710
display id: annotation2273710
version: 1
top level: BBa_K611059
location: https://synbiohub.org/public/igem/BBa_K611059/annotation2273710/range2273710/1
component: BBa_K611012

https://synbiohub.org/public/igem/BBa_K611059/annotation2273710/range2273710/1
type: Range
persistent identity: range2273710
display id: range2273710
version: 1
top level: BBa_K611059
direction: direction/reverse
start: 63
end: 1786
orientation: inline

BBa_K611049
type: ComponentDefinition
persistent identity: BBa K611049
display id: BBa_K611049
version: 1
was generated by: iGEM to SBOL conversion
description: pTet Variant #9
created: 2011-09-26T11:00:00Z
modified: 2015-05-08T01:12:53Z
mutable provenance: Mutagenic PCR of the Tet repressible promoter, R0040.
owned by: james
owned by: myers
mutable description: Parts K614021 through K614029 are variants of the wild type Tet repressible promoter, R0040. These parts were generated through three rounds of mutagenic PCR. Each of these variants differ in properties such as transcriptional strength and repressor binding affinity and have been well characterized. This can be very useful in tuning genetic circuits for specific outputs. These parts were characterized using K611012.
discontinued: false
dominant: false
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: This part does not have a termination sequence at the end. It is recommended that for optimal results, this part be used in a backbone that includes a terminator (such as pSB1C3).
star: false
creator: Nicholas Csicsery
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence annotation: t->c
sequence: https://synbiohub.org/public/igem/BBa_K611049_sequence/1

TetR 1
type: SequenceAnnotation
persistent identity: annotation2143830
display id: annotation2143830
version: 1
top level: BBa_K611049
location: https://synbiohub.org/public/igem/BBa_K611049/annotation2143830/range2143830/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K611049/annotation2143830/range2143830/1
type: Range
persistent identity: range2143830
display id: range2143830
version: 1
top level: BBa_K611049
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2143850
display id: annotation2143850
version: 1
top level: BBa_K611049
location: https://synbiohub.org/public/igem/BBa_K611049/annotation2143850/range2143850/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K611049/annotation2143850/range2143850/1
type: Range
persistent identity: range2143850
display id: range2143850
version: 1
top level: BBa_K611049
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation2143849
display id: annotation2143849
version: 1
top level: BBa_K611049
location: https://synbiohub.org/public/igem/BBa_K611049/annotation2143849/range2143849/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K611049/annotation2143849/range2143849/1
type: Range
persistent identity: range2143849
display id: range2143849
version: 1
top level: BBa_K611049
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2143851
display id: annotation2143851
version: 1
top level: BBa_K611049
location: https://synbiohub.org/public/igem/BBa_K611049/annotation2143851/range2143851/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K611049/annotation2143851/range2143851/1
type: Range
persistent identity: range2143851
display id: range2143851
version: 1
top level: BBa_K611049
direction: direction/forward
start: 43
end: 48
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation2143817
display id: annotation2143817
version: 1
top level: BBa_K611049
location: https://synbiohub.org/public/igem/BBa_K611049/annotation2143817/range2143817/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K611049/annotation2143817/range2143817/1
type: Range
persistent identity: range2143817
display id: range2143817
version: 1
top level: BBa_K611049
direction: direction/forward
start: 45
end: 45
orientation: inline

BBa_K611012
type: ComponentDefinition
persistent identity: BBa K611012
display id: BBa_K611012
version: 1
was generated by: iGEM to SBOL conversion
description: pTet Characterization Construct
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:53Z
mutable provenance: Built from existing parts in the registry though BioBrick assembly.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part is used to characterize mutants of BBa_R0040 (pTet). By adding a mutant of BBa_R0040 before this part, one can measure several of its properties through the expression of GFP. Certain characteristics of the mutant, such as the binding of the TetR repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.
discontinued: false
dominant: false
experience: experience/None
group u list: _783_
m user id: 0
owner id: 10185
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: There is no terminator at the end, so the part needs to be used in a plasmid that has a terminator after the BioBrick suffix (such as pSB1A3 or pSB1C3). This part contains the PBad promoter without AraC, thus, a strain that already includes AraC is recommended in order to induce PBad.
star: false
creator: Nicholas Csicsery
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_I13453
component: BBa_B0015
component: BBa_E0040
component: BBa_C0040
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence annotation: BBa_I13453
sequence annotation: BBa_C0040
sequence: https://synbiohub.org/public/igem/BBa_K611012_sequence/1

BBa_I13453
type: Component
persistent identity: component2268734
display id: component2268734
version: 1
top level: BBa_K611012
definition: BBa_I13453
access: public

BBa_B0015
type: Component
persistent identity: component2268733
display id: component2268733
version: 1
top level: BBa_K611012
definition: BBa_B0015
access: public

BBa_E0040
type: Component
persistent identity: component2268726
display id: component2268726
version: 1
top level: BBa_K611012
definition: GFP
access: public

BBa_C0040
type: Component
persistent identity: component2268740
display id: component2268740
version: 1
top level: BBa_K611012
definition: tetR
access: public

BBa_B0034
type: Component
persistent identity: component2268724
display id: component2268724
version: 1
top level: BBa_K611012
definition: BBa_B0034
access: public

BBa_B0034
type: Component
persistent identity: component2268736
display id: component2268736
version: 1
top level: BBa_K611012
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268724
display id: annotation2268724
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268724/range2268724/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611012/annotation2268724/range2268724/1
type: Range
persistent identity: range2268724
display id: range2268724
version: 1
top level: BBa_K611012
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2268726
display id: annotation2268726
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268726/range2268726/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_K611012/annotation2268726/range2268726/1
type: Range
persistent identity: range2268726
display id: range2268726
version: 1
top level: BBa_K611012
direction: direction/reverse
start: 19
end: 738
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2268733
display id: annotation2268733
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268733/range2268733/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K611012/annotation2268733/range2268733/1
type: Range
persistent identity: range2268733
display id: range2268733
version: 1
top level: BBa_K611012
direction: direction/reverse
start: 747
end: 875
orientation: inline

BBa_I13453
type: SequenceAnnotation
persistent identity: annotation2268734
display id: annotation2268734
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268734/range2268734/1
component: BBa_I13453

https://synbiohub.org/public/igem/BBa_K611012/annotation2268734/range2268734/1
type: Range
persistent identity: range2268734
display id: range2268734
version: 1
top level: BBa_K611012
direction: direction/reverse
start: 884
end: 1013
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2268736
display id: annotation2268736
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268736/range2268736/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K611012/annotation2268736/range2268736/1
type: Range
persistent identity: range2268736
display id: range2268736
version: 1
top level: BBa_K611012
direction: direction/reverse
start: 1022
end: 1033
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2268740
display id: annotation2268740
version: 1
top level: BBa_K611012
location: https://synbiohub.org/public/igem/BBa_K611012/annotation2268740/range2268740/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_K611012/annotation2268740/range2268740/1
type: Range
persistent identity: range2268740
display id: range2268740
version: 1
top level: BBa_K611012
direction: direction/reverse
start: 1040
end: 1724
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

BBa_I13453
type: ComponentDefinition
persistent identity: BBa I13453
display id: BBa_I13453
version: 1
was generated by: iGEM to SBOL conversion
description: Pbad promoter
created: 2005-05-24T11:00:00Z
modified: 2015-08-31T04:07:34Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PBad promoter from I0500 without AraC.
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_
m user id: 0
owner id: 253
owning group id: 6
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jkm
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence: https://synbiohub.org/public/igem/BBa_I13453_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_K611059_sequence/1
type: Sequence
persistent identity: BBa K611059 sequence
display id: BBa_K611059_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagacactgagcactactagagaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K611049_sequence/1
type: Sequence
persistent identity: BBa K611049 sequence
display id: BBa_K611049_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagacactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K611012_sequence/1
type: Sequence
persistent identity: BBa K611012 sequence
display id: BBa_K611012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I13453_sequence/1
type: Sequence
persistent identity: BBa I13453 sequence
display id: BBa_I13453_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z