##gff-version 3
##sequence-region BBa_K611062 1 3680
BBa_K611062	.	plasmid_vector	1	2948	.	+	0	ID=BBa_J61002;Name=BBa_J61002
BBa_K611062	.	CDS	27	707	.	+	0	ID=annotation1892273;Name=mRFP;Parent=BBa_J61002
BBa_K611062	.	primer_binding_site	948	967	.	+	0	ID=annotation1975768;Name=Verification reverse (VR) primer binding site;Parent=BBa_J61002
BBa_K611062	.	sequence_feature	2927	2948	.	+	0	ID=annotation1975715;Name=BioBrick prefix;Parent=BBa_J61002
BBa_K611062	.	stem_loop	752	795	.	+	0	ID=annotation1975713;Name=rrnB terminator;Parent=BBa_J61002
BBa_K611062	.	sequence_feature	1049	1731	.	+	0	ID=annotation1892275;Name=ColE1 origin;Parent=BBa_J61002
BBa_K611062	.	engineered_region	741	869	.	+	0	ID=annotation1892274;Name=BBa_B0015;Parent=BBa_J61002
BBa_K611062	.	sequence_feature	2	7	.	+	0	ID=annotation1975716;Name=SpeI;Parent=BBa_J61002
BBa_K611062	.	promoter	2728	2756	.	+	0	ID=annotation1892272;Name=beta-lactamase (ampR, bla) promoter;Parent=BBa_J61002
BBa_K611062	.	engineered_region	9	20	.	+	0	ID=annotation1975767;Name=BBa_B0034 RBS;Parent=BBa_J61002
BBa_K611062	.	sequence_feature	1052	1671	.	+	0	ID=annotation1975714;Name=pBR322 origin;Parent=BBa_J61002
BBa_K611062	.	sequence_feature	878	885	.	+	0	ID=annotation1975718;Name=NotI;Parent=BBa_J61002
BBa_K611062	.	primer_binding_site	2809	2828	.	+	0	ID=annotation1975769;Name=Verification forward (VF2) primer binding site;Parent=BBa_J61002
BBa_K611062	.	sequence_alteration	2941	2941	.	+	0	ID=annotation1975921;Name=Ambiguity; should be a T;Parent=BBa_J61002
BBa_K611062	.	CDS	1826	2686	.	+	0	ID=annotation1892276;Name=beta-lactamase ampicillin resistance marker;Parent=BBa_J61002
BBa_K611062	.	ribosome_entry_site	9	20	.	+	0	ID=annotation1975766;Name=RBS;Parent=BBa_J61002
BBa_K611062	.	sequence_feature	885	890	.	+	0	ID=annotation1975717;Name=PstI;Parent=BBa_J61002
BBa_K611062	.	engineered_region	2957	3680	.	+	0	ID=BBa_I13502;Name=BBa_I13502
BBa_K611062	.	ribosome_entry_site	2957	2968	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13502
BBa_K611062	.	conserved_region	2961	2964	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K611062	.	CDS	2975	3655	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_I13502
BBa_K611062	.	CDS	2975	3649	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K611062	.	engineered_tag	3656	3680	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K611062
tactagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtat
ggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgct
aaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaag
cttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttat
gaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaa
ctgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgta
tgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctga
agttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacc
tcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgct
gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt
tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
tactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcag
ctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagca
aaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaat
cgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgc
gctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatag
ctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagccc
gaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagcca
ctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacac
tagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggc
aaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaag
atcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatc
aaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttgg
tctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgac
tccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacg
ctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatcc
gcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttg
ccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcg
agttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggcc
gcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga
ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacggga
taataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatc
ttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcg
tttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcat
actcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttag
aaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatga
cattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgattt
ctggaattcgcggccgcatctagagtactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaag
agttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcg
tccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcc
ccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccgg
aaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaaga
cggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatg
ggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactga
aagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa
aaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgt
cactccaccggtgcttaataacgctgatagtgctagtgtagatcgc