LOCUS BBa_K611064 3729 bp DNA linear 22-Nov-2024 DEFINITION cI Lambda with TGATAA Mutated Stop Codon test construct ACCESSION BBa_K611064 VERSION BBa_K611064.1 FEATURES Location/Qualifiers promoter 2728..2756 /label=beta-lactamase (ampR, bla) promoter CDS 27..707 /label=mRFP misc_feature 741..869 /label=BBa_B0015 misc_feature 1049..1731 /label=ColE1 origin CDS 1826..2686 /label=beta-lactamase ampicillin resistance marker stem_loop 752..795 /label=rrnB terminator misc_feature 1052..1671 /label=pBR322 origin misc_feature 2927..2948 /label=BioBrick prefix misc_feature 2..7 /label=SpeI misc_feature 885..890 /label=PstI misc_feature 878..885 /label=NotI RBS 9..20 /label=RBS misc_feature 9..20 /label=BBa_B0034 RBS primer_bind 948..967 /label=Verification reverse (VR) primer binding site primer_bind 2809..2828 /label=Verification forward (VF2) primer binding site misc_feature 2941..2941 /label=Ambiguity; should be a T misc_feature 1..2948 /label=BBa_J61002 misc_feature 3705..3729 /label=Barcode misc_feature 3700..3700 /label=A->G CDS 2955..3699 /label=cI Lambda CDS 2955..3729 /label=BBa_K611060 ORIGIN 1 tactagtgaa agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca 61 tgcgtttcaa agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg 121 aaggtgaagg tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg 181 gtccgctgcc gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt 241 acgttaaaca cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca 301 aatgggaacg tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct 361 ccctgcaaga cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg 421 acggtccggt tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc 481 cggaagacgg tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc 541 actacgacgc tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg 601 cttacaaaac cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg 661 aacagtacga acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct 721 agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 781 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 841 accttcgggt gggcctttct gcgtttatat actagaagcg gccgctgcag gcttcctcgc 901 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg 961 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag 1021 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc 1081 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag 1141 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga 1201 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc 1261 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg 1321 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt 1381 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca 1441 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca 1501 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag 1561 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca 1621 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg 1681 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa 1741 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta 1801 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag 1861 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga 1921 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac 1981 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc 2041 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta 2101 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac 2161 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat 2221 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa 2281 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg 2341 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag 2401 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc 2461 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct 2521 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat 2581 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg 2641 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc 2701 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 2761 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 2821 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 2881 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 2941 atctagagta ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg 3001 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat 3061 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca 3121 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag 3181 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc 3241 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct 3301 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa 3361 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag 3421 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg 3481 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga 3541 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat 3601 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt 3661 ttggcgctgc aaacgacgaa aactacgctt tagtagcttg ataacgctga tagtgctagt 3721 gtagatcgc //