LOCUS BBa_K611081 3743 bp DNA linear 22-Dec-2024 DEFINITION BBa_C0051 GFP Test Construct ACCESSION BBa_K611081 VERSION BBa_K611081.1 FEATURES Location/Qualifiers misc_feature 1..6 /label=SpeI misc_feature 5..8 /label=conserved misc_feature 8..19 /label=BBa_B0034 CDS 26..745 /label=GFP protein misc_feature 26..745 /label=BBa_E0040 misc_feature 755..883 /label=BBa_B0015 stem_loop 766..809 /label=rrnB terminator misc_feature 892..935 /label=NotI misc_feature 935..940 /label=PstI primer_bind 998..1017 /label=Verification reverse (VR) primer binding site misc_feature 1099..1781 /label=ColE1 origin misc_feature 1102..1721 /label=pBR322 origin CDS 1876..2736 /label=beta-lactamase ampicillin resistance marker promoter 2778..2806 /label=beta-lactamase (ampR, bla) promoter primer_bind 2859..2878 /label=Verification forward (VF2) primer binding site misc_feature 2941..2962 /label=BioBrick prefix misc_feature 2968..3717 /label=BBa_C0051 CDS 2968..3717 /label=cI lambda misc_feature 3719..3743 /label=barcode ORIGIN 1 actagtgaaa gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt 61 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga 121 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa 181 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc 241 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta 301 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt 361 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga 421 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat 481 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga 541 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc 601 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa 661 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg 721 catggatgaa ctatacaaat aataatacta gagtccaggc atcaaataaa acgaaaggct 781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga agcggccgct 901 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc 961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa 1021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt 1081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg 1141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg 1201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag 1261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc 1321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa 1381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg 1441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc 1501 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac 1561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg 1621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt 1681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt 1741 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa 1801 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga 1861 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt 1921 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg 1981 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga 2041 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga 2101 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg 2161 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc 2221 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc 2281 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca 2341 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac 2401 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg 2461 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc 2521 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg 2581 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac 2641 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat 2701 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata 2761 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa 2821 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg 2881 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg 2941 gaattcgcgg ccgcatctag agtactagat gagcacaaaa aagaaaccat taacacaaga 3001 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg 3061 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt 3121 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa 3181 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc 3241 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca 3301 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt 3361 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat 3421 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc 3481 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac 3541 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta 3601 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg 3661 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg 3721 ctgatagtgc tagtgtagat cgc //