LOCUS       BBa_K611082             2968 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP (BBa_E0040) Screening Plasmid
ACCESSION   BBa_K611082
VERSION     BBa_K611082.1  
FEATURES             Location/Qualifiers
     misc_feature    1..2968
                     /label=BBa_K611081
     misc_feature    1..6
                     /label=SpeI
     misc_feature    5..8
                     /label=conserved
     misc_feature    8..19
                     /label=BBa_B0034
     misc_feature    26..745
                     /label=BBa_E0040
     CDS             26..745
                     /label=GFP protein
     misc_feature    755..883
                     /label=BBa_B0015
     stem_loop       766..809
                     /label=rrnB terminator
     misc_feature    892..935
                     /label=NotI
     misc_feature    935..940
                     /label=PstI
     primer_bind     998..1017
                     /label=Verification reverse (VR) primer binding site
     misc_feature    1099..1781
                     /label=ColE1 origin
     misc_feature    1102..1721
                     /label=pBR322 origin
     CDS             1876..2736
                     /label=beta-lactamase ampicillin resistance marker
     promoter        2778..2806
                     /label=beta-lactamase (ampR, bla) promoter
     primer_bind     2859..2878
                     /label=Verification forward (VF2) primer binding site
     misc_feature    2941..2962
                     /label=BioBrick prefix
ORIGIN
        1 actagtgaaa gaggagaaat actagatgcg taaaggagaa gaacttttca ctggagttgt
       61 cccaattctt gttgaattag atggtgatgt taatgggcac aaattttctg tcagtggaga
      121 gggtgaaggt gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa
      181 actacctgtt ccatggccaa cacttgtcac tactttcggt tatggtgttc aatgctttgc
      241 gagataccca gatcatatga aacagcatga ctttttcaag agtgccatgc ccgaaggtta
      301 tgtacaggaa agaactatat ttttcaaaga tgacgggaac tacaagacac gtgctgaagt
      361 caagtttgaa ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga
      421 agatggaaac attcttggac acaaattgga atacaactat aactcacaca atgtatacat
      481 catggcagac aaacaaaaga atggaatcaa agttaacttc aaaattagac acaacattga
      541 agatggaagc gttcaactag cagaccatta tcaacaaaat actccaattg gcgatggccc
      601 tgtcctttta ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa
      661 cgaaaagaga gaccacatgg tccttcttga gtttgtaaca gctgctggga ttacacatgg
      721 catggatgaa ctatacaaat aataatacta gagtccaggc atcaaataaa acgaaaggct
      781 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
      841 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga agcggccgct
      901 gcaggcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
      961 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1021 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1081 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1141 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1201 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1261 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1321 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1381 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1441 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1501 taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac
     1561 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1621 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1681 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     1741 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     1801 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     1861 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     1921 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     1981 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2041 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2101 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2161 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2221 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2281 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2341 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2401 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2461 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2521 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2581 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2641 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     2701 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     2761 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     2821 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     2881 tatcacgagg cagaatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
     2941 gaattcgcgg ccgcatctag agtactag
//