LOCUS BBa_K611083 3727 bp DNA linear 27-Sep-2024 DEFINITION BBa_C0051 Test Construct +1(TSS) delete ACCESSION BBa_K611083 VERSION BBa_K611083.1 FEATURES Location/Qualifiers misc_feature 1..6 /label=SpeI misc_feature 1..2947 /label=BBa_J61002 misc_feature 8..19 /label=BBa_B0034 RBS CDS 26..706 /label=mRFP misc_feature 740..868 /label=BBa_B0015 stem_loop 751..794 /label=rrnB terminator misc_feature 877..884 /label=NotI misc_feature 884..889 /label=PstI primer_bind 947..966 /label=Verification reverse (VR) primer binding site misc_feature 1048..1730 /label=ColE1 origin misc_feature 1051..1670 /label=pBR322 origin CDS 1825..2685 /label=beta-lactamase ampicillin resistance marker promoter 2727..2755 /label=beta-lactamase (ampR, bla) promoter primer_bind 2808..2827 /label=Verification forward (VF2) primer binding site misc_feature 2925..2946 /label=BioBrick prefix misc_feature 2940..2940 /label=Ambiguity; should be a T misc_feature 2956..3727 /label=BBa_K611063 misc_feature 2956..3663 /label=Truncated cI Lamda Missing ATG Start Codon misc_feature 3664..3696 /label=LVA misc_feature 3703..3727 /label=Barcode ORIGIN 1 actagtgaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat 61 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga 121 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg 181 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta 241 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa 301 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc 361 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga 421 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc 481 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca 541 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc 601 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga 661 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta 721 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg 781 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca 841 ccttcgggtg ggcctttctg cgtttatata ctagaagcgg ccgctgcagg cttcctcgct 901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 1021 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 1081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 1141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 1201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 1261 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 1321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 1381 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 1441 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 1501 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 1561 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 1621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 1681 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 1741 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 1801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 1861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 1921 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 1981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 2041 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 2101 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 2161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 2221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 2281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 2341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 2401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 2461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 2521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 2581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 2641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 2701 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 2761 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 2821 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa 2881 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgca 2941 tctagagtac tagagagcac aaaaaagaaa ccattaacac aagagcagct tgaggacgca 3001 cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc ccaggaatct 3061 gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa tggcatcaat 3121 gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag cgttgaagaa 3181 tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag tatgcagccg 3241 tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg gatgttctca 3301 cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac aaccaaaaaa 3361 gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc accaacaggc 3421 tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca ggctgttgag 3481 ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa gaaactgatc 3541 agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat gatcccatgc 3601 aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga agagacgttt 3661 ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacgctgata gtgctagtgt 3721 agatcgc //