##gff-version 3
##sequence-region BBa_K611083 1 3727
BBa_K611083	.	primer_binding_site	2808	2827	.	+	0	ID=annotation2180432;Name=Verification forward (VF2) primer binding site
BBa_K611083	.	sequence_feature	1048	1730	.	+	0	ID=annotation2180428;Name=ColE1 origin
BBa_K611083	.	sequence_feature	877	884	.	+	0	ID=annotation2180425;Name=NotI
BBa_K611083	.	promoter	2727	2755	.	+	0	ID=annotation2180431;Name=beta-lactamase (ampR, bla) promoter
BBa_K611083	.	engineered_tag	3703	3727	.	+	0	ID=annotation2180438;Name=Barcode
BBa_K611083	.	sequence_feature	1051	1670	.	+	0	ID=annotation2180429;Name=pBR322 origin
BBa_K611083	.	sequence_feature	2925	2946	.	+	0	ID=annotation2180433;Name=BioBrick prefix
BBa_K611083	.	engineered_region	1	2947	.	+	0	ID=annotation2180420;Name=BBa_J61002
BBa_K611083	.	sequence_alteration	2940	2940	.	+	0	ID=annotation2180434;Name=Ambiguity; should be a T
BBa_K611083	.	engineered_region	740	868	.	+	0	ID=annotation2180423;Name=BBa_B0015
BBa_K611083	.	CDS	1825	2685	.	+	0	ID=annotation2180430;Name=beta-lactamase ampicillin resistance marker
BBa_K611083	.	sequence_feature	2956	3663	.	+	0	ID=annotation2180436;Name=Truncated cI Lamda Missing ATG Start Codon
BBa_K611083	.	CDS	26	706	.	+	0	ID=annotation2180422;Name=mRFP
BBa_K611083	.	stem_loop	751	794	.	+	0	ID=annotation2180424;Name=rrnB terminator
BBa_K611083	.	engineered_region	2956	3727	.	+	0	ID=annotation2180435;Name=BBa_K611063
BBa_K611083	.	sequence_feature	1	6	.	+	0	ID=annotation2180419;Name=SpeI
BBa_K611083	.	engineered_region	8	19	.	+	0	ID=annotation2180421;Name=BBa_B0034 RBS
BBa_K611083	.	primer_binding_site	947	966	.	+	0	ID=annotation2180427;Name=Verification reverse (VR) primer binding site
BBa_K611083	.	sequence_feature	884	889	.	+	0	ID=annotation2180426;Name=PstI
BBa_K611083	.	tag	3664	3696	.	+	0	ID=annotation2180437;Name=LVA
>BBa_K611083
actagtgaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatg
gaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgcta
aactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagc
ttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatg
aacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaac
tgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtat
gtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaa
gttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacct
cccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctg
atagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgtt
ttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatat
actagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaa
aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg
ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg
accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac
tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca
aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaga
tcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca
aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggt
ctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgact
ccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgc
tcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccg
cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgc
cattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcga
gttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccg
cagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgac
tggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat
aataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct
taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgt
ttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcata
ctcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttaga
aaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgac
attaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttc
tggaattcgcggccgcatctagagtactagagagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacg
tcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatg
gggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattc
tcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagcc
gtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaaccttt
accaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggta
attccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgt
tgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcag
gtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcg
ctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtg
ctagtgtagatcgc