LOCUS       BBa_K611084             3008 bp    DNA     linear       26-Dec-2024
DEFINITION  LVA∆5'11 (BBa_K611072) with a +1 deletion (TSS deletion)
ACCESSION   BBa_K611084
VERSION     BBa_K611084.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=SpeI
     misc_feature    1..2947
                     /label=BBa_J61002
     misc_feature    8..19
                     /label=BBa_B0034 RBS
     CDS             26..706
                     /label=mRFP
     misc_feature    740..868
                     /label=BBa_B0015
     stem_loop       751..794
                     /label=rrnB terminator
     misc_feature    877..884
                     /label=NotI
     misc_feature    884..889
                     /label=PstI
     primer_bind     947..966
                     /label=Verification reverse (VR) primer binding site
     misc_feature    1048..1730
                     /label=ColE1 origin
     misc_feature    1051..1670
                     /label=pBR322 origin
     CDS             1825..2685
                     /label=beta-lactamase ampicillin resistance marker
     promoter        2727..2755
                     /label=beta-lactamase (ampR, bla) promoter
     primer_bind     2808..2827
                     /label=Verification forward (VF2) primer binding site
     misc_feature    2926..2947
                     /label=BioBrick prefix
     misc_feature    2940..2940
                     /label=Ambiguity; should be a T
     misc_feature    2956..2977
                     /label=LVA 5
     misc_feature    2956..3008
                     /label=BBa_K611095
     misc_feature    2984..3008
                     /label=Barcode
ORIGIN
        1 actagtgaaa gaggagaaat actagatggc ttcctccgaa gacgttatca aagagttcat
       61 gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga
      121 aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg
      181 tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta
      241 cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa
      301 atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc
      361 cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga
      421 cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc
      481 ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca
      541 ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc
      601 ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga
      661 acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct gatagtgcta
      721 gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
      781 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
      841 ccttcgggtg ggcctttctg cgtttatata ctagaagcgg ccgctgcagg cttcctcgct
      901 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
      961 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     1021 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     1081 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     1141 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     1201 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     1261 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     1321 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     1381 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     1441 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     1501 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     1561 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     1621 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     1681 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     1741 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     1801 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     1861 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     1921 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     1981 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     2041 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     2101 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     2161 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     2221 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     2281 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     2341 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     2401 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     2461 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     2521 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     2581 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     2641 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     2701 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat
     2761 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt
     2821 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa
     2881 tttcagataa aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgca
     2941 tctagagtac tagagcgaaa actacgcttt agtagcttaa taacgctgat agtgctagtg
     3001 tagatcgc
//