##gff-version 3
##sequence-region BBa_K611085 1 3749
BBa_K611085	.	engineered_region	1	2968	.	+	0	ID=BBa_K611082;Name=BBa_K611082
BBa_K611085	.	sequence_feature	2941	2962	.	+	0	ID=annotation2179881;Name=BioBrick prefix;Parent=BBa_K611082
BBa_K611085	.	sequence_feature	1102	1721	.	+	0	ID=annotation2179877;Name=pBR322 origin;Parent=BBa_K611082
BBa_K611085	.	CDS	26	745	.	+	0	ID=annotation2179870;Name=GFP protein;Parent=BBa_K611082
BBa_K611085	.	primer_binding_site	998	1017	.	+	0	ID=annotation2179875;Name=Verification reverse (VR) primer binding site;Parent=BBa_K611082
BBa_K611085	.	sequence_feature	892	935	.	+	0	ID=annotation2179873;Name=NotI;Parent=BBa_K611082
BBa_K611085	.	sequence_feature	935	940	.	+	0	ID=annotation2179874;Name=PstI;Parent=BBa_K611082
BBa_K611085	.	engineered_region	8	19	.	+	0	ID=annotation2179868;Name=BBa_B0034;Parent=BBa_K611082
BBa_K611085	.	stem_loop	766	809	.	+	0	ID=annotation2179872;Name=rrnB terminator;Parent=BBa_K611082
BBa_K611085	.	primer_binding_site	2859	2878	.	+	0	ID=annotation2179880;Name=Verification forward (VF2) primer binding site;Parent=BBa_K611082
BBa_K611085	.	sequence_feature	1099	1781	.	+	0	ID=annotation2179876;Name=ColE1 origin;Parent=BBa_K611082
BBa_K611085	.	engineered_region	26	745	.	+	0	ID=annotation2179869;Name=BBa_E0040;Parent=BBa_K611082
BBa_K611085	.	engineered_region	1	2968	.	+	0	ID=annotation2179865;Name=BBa_K611081;Parent=BBa_K611082
BBa_K611085	.	engineered_region	755	883	.	+	0	ID=annotation2179871;Name=BBa_B0015;Parent=BBa_K611082
BBa_K611085	.	sequence_feature	1	6	.	+	0	ID=annotation2179866;Name=SpeI;Parent=BBa_K611082
BBa_K611085	.	CDS	1876	2736	.	+	0	ID=annotation2179878;Name=beta-lactamase ampicillin resistance marker;Parent=BBa_K611082
BBa_K611085	.	conserved_region	5	8	.	+	0	ID=annotation2179867;Name=conserved;Parent=BBa_K611082
BBa_K611085	.	promoter	2778	2806	.	+	0	ID=annotation2179879;Name=beta-lactamase (ampR, bla) promoter;Parent=BBa_K611082
BBa_K611085	.	CDS	2975	3749	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K611085	.	tag	3686	3718	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K611085	.	CDS	2978	3685	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K611085	.	engineered_tag	3725	3749	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_K611085
actagtgaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatta
gatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctta
aatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctt
tgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaact
atatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcg
agttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgt
atacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgtt
caactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcca
cacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggat
tacacatggcatggatgaactatacaaataataatactagagtccaggcatcaaataaaacgaaaggctcagtcgaaag
actgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcc
tttctgcgtttatatactagaagcggccgctgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcg
gcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtga
gcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacg
agcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctgg
aagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtg
gcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaac
cccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgcc
actggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcct
aactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggta
gctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaa
aggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttg
gtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat
atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatc
catagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgata
ccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtc
ctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagttt
gcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcc
caacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtca
gaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaag
atgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccg
gcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaa
aactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttt
tactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaa
tgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatat
ttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaac
cattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttc
gctaaggatgatttctggaattcgcggccgcatctagagtactagtactagatgagcacaaaaaagaaaccattaacac
aagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatc
tgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataac
gccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgt
atgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgtt
ctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgca
ttctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattc
tcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaa
actgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgt
tccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttag
tagcttaataacgctgatagtgctagtgtagatcgc