BBa_K613007
1
BBa_K613007
T7 promoter family member (LacI repressed)
2011-09-19T11:00:00Z
2015-05-08T01:12:54Z
synthetic
Parts K613007 through K613012 are T7 promoter variants containing the lac operator just downstream of the T7 promoter. K613007 contains the T7 promoter wildtype sequence. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.
false
false
_785_
0
7125
9
Not in stock
false
Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419???10430 (2000).
false
Alessandro Ferrari, Vincent Zimmern, Nadine Guenat, Henrike Niederholtmeyer
annotation2136017
1
T7 promoter
range2136017
1
30
52
annotation2136018
1
lac operator
range2136018
1
55
75
annotation2136019
1
RBS
range2136019
1
107
112
BBa_K613007_sequence
1
gaagcgagaggatcttaaggctagagtactaatacgactcactatagggagaggaattgtgagcggataacaattcccactagaaataattttgtttaacttaagaaggaggaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z