BBa_K613014 1 BBa_K613014 TetR V36FW43S mutant 2011-09-19T11:00:00Z 2015-05-08T01:12:54Z The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36FW43S mutation by site-directed mutagenesis. The V36F mutation had already been described by Krueger et al (2007). This is a TetR mutant, who carries the VF36WS43 mutation. false false _785_ 0 9696 9 Not in stock false The mutation was introduced by site-directed mutagenesis. false Lilia Salimova annotation2136976 1 V to F range2136976 1 106 108 annotation2136977 1 W to S range2136977 1 127 129 BBa_K613014_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtttcgagcagcctacattgtattcgcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z