BBa_K613020 1 BBa_K613020 TetR P39Q Y42M L197S mutant 2011-09-19T11:00:00Z 2015-05-08T01:12:54Z The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998). This is a TetR mutant with a PQ39YM42LS197 triple mutation. false false _785_ 0 9696 9 Not in stock false The mutation was introduced by site-directed mutagenesis false Nadine Guenat annotation2137211 1 L197S range2137211 1 589 591 annotation2137209 1 Y42M range2137209 1 124 126 annotation2137208 1 P39Q range2137208 1 115 117 BBa_K613020_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcaaacattgatgtggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatcagaaaaacaacttaaatgtgaaagtgggtct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z