BBa_K613020
1
BBa_K613020
TetR P39Q Y42M L197S mutant
2011-09-19T11:00:00Z
2015-05-08T01:12:54Z
The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the V36F mutation by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998).
This is a TetR mutant with a PQ39YM42LS197 triple mutation.
false
false
_785_
0
9696
9
Not in stock
false
The mutation was introduced by site-directed mutagenesis
false
Nadine Guenat
annotation2137211
1
L197S
range2137211
1
589
591
annotation2137209
1
Y42M
range2137209
1
124
126
annotation2137208
1
P39Q
range2137208
1
115
117
BBa_K613020_sequence
1
atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcaaacattgatgtggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggatcagaaaaacaacttaaatgtgaaagtgggtct
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z