BBa_K613021 1 BBa_K613021 TetR PQ39YM42LP52 mutant 2011-09-19T11:00:00Z 2015-05-08T01:12:54Z The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced mutations by site-directed mutagenesis. Parts of this mutation had already been described by Helbl and Hillen (1998). This is a TetR mutant with the PQ39YM42LP52 triple mutation false false _785_ 0 9696 9 Not in stock false The mutation was introduced by site-directed mutagenesis false Nadine Guenat annotation2137213 1 P39Q range2137213 1 115 117 annotation2137216 1 L52P range2137216 1 154 156 annotation2137215 1 Y42M range2137215 1 124 126 BBa_K613021_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcaaacattgatgtggcatgtaaaaaataagcgggctttgcccgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtct igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z