BBa_K613022 1 BBa_K613022 TetR E37A P39K mutant 2011-09-19T11:00:00Z 2015-05-08T01:12:54Z The TetR gene was PCR-amplified from the Repressilator plasmid. We then introduced the mutation by site-directed mutagenesis. This is a TetR mutant with the EA37PK39 double mutation false false _785_ 0 9696 9 Not in stock false The mutation was introduced by site-directed mutagenesis false Nadine Guenat, Alina Isakova annotation2137091 1 BBa_K613022 range2137091 1 1 624 annotation2137090 1 P39K range2137090 1 115 117 annotation2137089 1 E37A range2137089 1 109 111 BBa_K613022_sequence 1 atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagcgcagaagacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z