LOCUS       BBa_K614000              882 bp    DNA     linear       22-Dec-2024
DEFINITION  PyeaR promoter with RFP reporter
ACCESSION   BBa_K614000
VERSION     BBa_K614000.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     RBS             109..123
                     /label=RBS-1\Strong
     RBS             116..120
                     /label=RBS
     misc_feature    109..123
                     /label=BBa_B0030
     RBS             109..123
                     /label=BBa_B0030
     CDS             130..804
                     /label=mrfp1
     misc_feature    811..835
                     /label=Help:Barcodes
     CDS             130..835
                     /label=BBa_E1010
     misc_feature    844..882
                     /label=B1006
     stem_loop       853..874
                     /label=modified thr terminator
     polyA_site      875..882
                     /label=PolyA
     polyA_site      844..852
                     /label=PolyA
     terminator      844..882
                     /label=BBa_B1006
     misc_feature    109..882
                     /label=BBa_K081014
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagat taaagaggag
      121 aaatactaga tggcttcctc cgaagacgtt atcaaagagt tcatgcgttt caaagttcgt
      181 atggaaggtt ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg
      241 tacgaaggta cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct
      301 tgggacatcc tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct
      361 gacatcccgg actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg
      421 aacttcgaag acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag
      481 ttcatctaca aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag
      541 aaaaaaacca tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg
      601 aaaggtgaaa tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt
      661 aaaaccacct acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc
      721 aaactggaca tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct
      781 gaaggtcgtc actccaccgg tgcttaataa cgctgatagt gctagtgtag atcgctacta
      841 gagaaaaaaa aaccccgccc ctgacagggc ggggtttttt tt
//