LOCUS       BBa_K614001             1363 bp    DNA     linear       22-Dec-2024
DEFINITION  Nitrate responsive promoter with amplifier
ACCESSION   BBa_K614001
VERSION     BBa_K614001.1  
FEATURES             Location/Qualifiers
     misc_feature    50..65
                     /label=NarL-binding region
     misc_feature    58..80
                     /label=NsrR-binding region
     misc_feature    66..71
                     /label=promoter -35 region
     misc_feature    89..94
                     /label=promoter -10 region
     promoter        1..100
                     /label=BBa_K216005
     misc_feature    109..120
                     /label=B0034
     misc_feature    127..127
                     /label=P2 ogr
     CDS             127..345
                     /label=P2 ogr
     misc_feature    109..345
                     /label=BBa_I746350
     stem_loop       365..408
                     /label=stem_loop
     misc_feature    354..433
                     /label=BBa_B0010
     terminator      354..433
                     /label=BBa_B0010
     stem_loop       449..468
                     /label=T7 TE
     misc_feature    475..475
                     /label=stop
     polyA_site      469..482
                     /label=polya
     misc_feature    442..482
                     /label=BBa_B0012
     terminator      442..482
                     /label=BBa_B0012
     promoter        491..581
                     /label=PF
     promoter        491..581
                     /label=BBa_I746360
     misc_feature    109..581
                     /label=BBa_K274370
     RBS             590..604
                     /label=RBS-1\Strong
     RBS             597..601
                     /label=RBS
     misc_feature    590..604
                     /label=BBa_B0030
     RBS             590..604
                     /label=BBa_B0030
     CDS             611..1285
                     /label=mrfp1
     misc_feature    1292..1316
                     /label=Help:Barcodes
     CDS             611..1316
                     /label=BBa_E1010
     misc_feature    1325..1363
                     /label=B1006
     stem_loop       1334..1355
                     /label=modified thr terminator
     polyA_site      1356..1363
                     /label=PolyA
     polyA_site      1325..1333
                     /label=PolyA
     terminator      1325..1363
                     /label=BBa_B1006
     misc_feature    590..1363
                     /label=BBa_K081014
ORIGIN
        1 ttcccatcta taatcctccc tgattcttcg ctgatatggt gctaaaaagt aaccaataaa
       61 tggtatttaa aatgcaaatt atcaggcgta ccctgaaacg tactagagaa agaggagaaa
      121 tactagatgt ttcattgtcc tttatgccag catgccgcac atgcgcgtac aagtcgctat
      181 atcactgaca cgacaaaaga gcgttatcat cagtgccaga acgtgaattg cagcgccacg
      241 ttcatcactt atgagtcggt acagcgatac atcgtgaagc cgggagaagt ccacgccgta
      301 aggccgcacc cgttgccatc agggcagcaa attatgtgga tgtaatacta gagccaggca
      361 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      421 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      481 tatactagag ttgcccgcct tttctttacc ggtggttgtg ctgtcgatta gccaaccggg
      541 acaaatagcc tgacatctcc ggcgcaactg aaaataccac ttactagaga ttaaagagga
      601 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg
      661 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc
      721 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc
      781 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc
      841 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat
      901 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga
      961 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca
     1021 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct
     1081 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt
     1141 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat
     1201 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc
     1261 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact
     1321 agagaaaaaa aaaccccgcc cctgacaggg cggggttttt ttt
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