##gff-version 3
##sequence-region BBa_K614001 1 1363
BBa_K614001	.	engineered_region	590	1363	.	+	0	ID=BBa_K081014;Name=BBa_K081014
BBa_K614001	.	CDS	611	1316	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K614001	.	engineered_tag	1292	1316	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K614001	.	CDS	611	1285	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K614001	.	ribosome_entry_site	590	604	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K614001	.	ribosome_entry_site	590	604	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K614001	.	engineered_region	590	604	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K614001	.	ribosome_entry_site	597	601	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K614001	.	terminator	1325	1363	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K614001	.	polyA_site	1325	1333	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K614001	.	polyA_site	1356	1363	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K614001	.	engineered_region	1325	1363	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K614001	.	stem_loop	1334	1355	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K614001	.	engineered_region	109	581	.	+	0	ID=BBa_K274370;Name=BBa_K274370
BBa_K614001	.	promoter	491	581	.	+	0	ID=BBa_I746360;Name=BBa_I746360;Parent=BBa_K274370
BBa_K614001	.	promoter	491	581	.	+	0	ID=annotation1943783;Name=PF;Parent=BBa_I746360
BBa_K614001	.	engineered_region	109	345	.	+	0	ID=BBa_I746350;Name=BBa_I746350;Parent=BBa_K274370
BBa_K614001	.	start_codon	127	127	.	+	0	ID=annotation1943866;Name=P2 ogr;Parent=BBa_I746350
BBa_K614001	.	engineered_region	109	120	.	+	0	ID=annotation1943865;Name=B0034;Parent=BBa_I746350
BBa_K614001	.	CDS	127	345	.	+	0	ID=annotation1943867;Name=P2 ogr;Parent=BBa_I746350
BBa_K614001	.	terminator	354	433	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K274370
BBa_K614001	.	engineered_region	354	433	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K614001	.	stem_loop	365	408	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K614001	.	terminator	442	482	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K274370
BBa_K614001	.	stem_loop	449	468	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K614001	.	stop_codon	475	475	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K614001	.	engineered_region	442	482	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K614001	.	polyA_site	469	482	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K614001	.	promoter	1	100	.	+	0	ID=BBa_K216005;Name=BBa_K216005
BBa_K614001	.	non_covalent_binding_site	66	71	.	+	0	ID=annotation2027074;Name=promoter -35 region;Parent=BBa_K216005
BBa_K614001	.	non_covalent_binding_site	58	80	.	+	0	ID=annotation2027073;Name=NsrR-binding region;Parent=BBa_K216005
BBa_K614001	.	non_covalent_binding_site	50	65	.	+	0	ID=annotation2027072;Name=NarL-binding region;Parent=BBa_K216005
BBa_K614001	.	non_covalent_binding_site	89	94	.	+	0	ID=annotation2027075;Name=promoter -10 region;Parent=BBa_K216005
>BBa_K614001
ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaat
tatcaggcgtaccctgaaacgtactagagaaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgc
acatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgcc
acgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgc
catcagggcagcaaattatgtggatgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctga
catctccggcgcaactgaaaataccacttactagagattaaagaggagaaatactagatggcttcctccgaagacgtta
tcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtga
aggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatc
ctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtcct
tcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccct
gcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaa
accatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctga
aactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgc
ttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaa
ggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccct
gacagggcggggtttttttt