BBa_K614001
type: ComponentDefinition
persistent identity: BBa K614001
display id: BBa_K614001
version: 1
was generated by: iGEM to SBOL conversion
description: Nitrate responsive promoter with amplifier
created: 2011-10-08T11:00:00Z
modified: 2015-05-08T01:12:54Z
mutable provenance: This is a combination of existing parts in the registry.
owned by: james
owned by: myers
mutable description: This plasmid should amplify the signal generated by the PyeaR promoter using amplifier BBa_K274370 from Cambridge 2009.
discontinued: false
dominant: false
group u list: _786_
m user id: 0
owner id: 9218
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None
star: false
creator: Steven Allen
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K081014
component: BBa_K274370
component: BBa_K216005
sequence annotation: BBa_K216005
sequence annotation: BBa_K274370
sequence annotation: BBa_K081014
sequence: https://synbiohub.org/public/igem/BBa_K614001_sequence/1

BBa_K081014
type: Component
persistent identity: component2273794
display id: component2273794
version: 1
top level: BBa_K614001
definition: BBa_K081014
access: public

BBa_K274370
type: Component
persistent identity: component2273782
display id: component2273782
version: 1
top level: BBa_K614001
definition: BBa_K274370
access: public

BBa_K216005
type: Component
persistent identity: component2273769
display id: component2273769
version: 1
top level: BBa_K614001
definition: BBa_K216005
access: public

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2273769
display id: annotation2273769
version: 1
top level: BBa_K614001
location: https://synbiohub.org/public/igem/BBa_K614001/annotation2273769/range2273769/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K614001/annotation2273769/range2273769/1
type: Range
persistent identity: range2273769
display id: range2273769
version: 1
top level: BBa_K614001
direction: direction/reverse
start: 1
end: 100
orientation: inline

BBa_K274370
type: SequenceAnnotation
persistent identity: annotation2273782
display id: annotation2273782
version: 1
top level: BBa_K614001
location: https://synbiohub.org/public/igem/BBa_K614001/annotation2273782/range2273782/1
component: BBa_K274370

https://synbiohub.org/public/igem/BBa_K614001/annotation2273782/range2273782/1
type: Range
persistent identity: range2273782
display id: range2273782
version: 1
top level: BBa_K614001
direction: direction/reverse
start: 109
end: 581
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2273794
display id: annotation2273794
version: 1
top level: BBa_K614001
location: https://synbiohub.org/public/igem/BBa_K614001/annotation2273794/range2273794/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K614001/annotation2273794/range2273794/1
type: Range
persistent identity: range2273794
display id: range2273794
version: 1
top level: BBa_K614001
direction: direction/reverse
start: 590
end: 1363
orientation: inline

BBa_K081014
type: ComponentDefinition
persistent identity: BBa K081014
display id: BBa_K081014
version: 1
was generated by: iGEM to SBOL conversion
description: RFP protein generator - PoPS->RFP
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: RBS: RFP: T:
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PoPS in -> RFP out Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
discontinued: false
dominant: true
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_B1006
component: BBa_E1010
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081014_sequence/1

BBa_B0030
type: Component
persistent identity: component2246146
display id: component2246146
version: 1
top level: BBa_K081014
definition: BBa_B0030
access: public

BBa_B1006
type: Component
persistent identity: component2246155
display id: component2246155
version: 1
top level: BBa_K081014
definition: BBa_B1006
access: public

BBa_E1010
type: Component
persistent identity: component2246150
display id: component2246150
version: 1
top level: BBa_K081014
definition: mRFP1
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246146
display id: annotation2246146
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
type: Range
persistent identity: range2246146
display id: range2246146
version: 1
top level: BBa_K081014
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246150
display id: annotation2246150
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
type: Range
persistent identity: range2246150
display id: range2246150
version: 1
top level: BBa_K081014
direction: direction/reverse
start: 22
end: 727
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246155
display id: annotation2246155
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
type: Range
persistent identity: range2246155
display id: range2246155
version: 1
top level: BBa_K081014
direction: direction/reverse
start: 736
end: 774
orientation: inline

BBa_K216005
type: ComponentDefinition
persistent identity: BBa K216005
display id: BBa_K216005
version: 1
was generated by: iGEM to SBOL conversion
description: PyeaR promoter, responsive to nitrate, nitrite and nitric oxide
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: BioBricked from E. coli K-12 genomic DNA by the Edinburgh 2009 iGEM team.
owned by: james
owned by: myers
mutable description: PyeaR promoter. This is the promoter of the Escherichia coli yeaR/yoaG operon (see Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in ''Escherichia coli'' K-12. J. Bacteriol. 189, 7539-7548). Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No special considerations.
star: false
creator: Edinburgh iGEM 2009
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NarL-binding region
sequence annotation: NsrR-binding region
sequence annotation: promoter -35 region
sequence annotation: promoter -10 region
sequence: https://synbiohub.org/public/igem/BBa_K216005_sequence/1

NarL-binding region
type: SequenceAnnotation
persistent identity: annotation2027072
display id: annotation2027072
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
type: Range
persistent identity: range2027072
display id: range2027072
version: 1
top level: BBa_K216005
direction: direction/forward
start: 50
end: 65
orientation: inline

NsrR-binding region
type: SequenceAnnotation
persistent identity: annotation2027073
display id: annotation2027073
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
type: Range
persistent identity: range2027073
display id: range2027073
version: 1
top level: BBa_K216005
direction: direction/forward
start: 58
end: 80
orientation: inline

promoter -35 region
type: SequenceAnnotation
persistent identity: annotation2027074
display id: annotation2027074
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
type: Range
persistent identity: range2027074
display id: range2027074
version: 1
top level: BBa_K216005
direction: direction/forward
start: 66
end: 71
orientation: inline

promoter -10 region
type: SequenceAnnotation
persistent identity: annotation2027075
display id: annotation2027075
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
type: Range
persistent identity: range2027075
display id: range2027075
version: 1
top level: BBa_K216005
direction: direction/forward
start: 89
end: 94
orientation: inline

BBa_K274370
type: ComponentDefinition
persistent identity: BBa K274370
display id: BBa_K274370
version: 1
was generated by: iGEM to SBOL conversion
description: PoPS -> P2 ogr - PF promoter -> PoPS
created: 2009-10-18T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: n/a
owned by: james
owned by: myers
mutable description: n/a
discontinued: false
dominant: false
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5448
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: n/a
star: false
creator: Vivian Mullin
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0010
component: BBa_I746350
component: BBa_I746360
component: BBa_B0012
sequence annotation: BBa_I746350
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_I746360
sequence: https://synbiohub.org/public/igem/BBa_K274370_sequence/1

BBa_B0010
type: Component
persistent identity: component2055283
display id: component2055283
version: 1
top level: BBa_K274370
definition: BBa_B0010
access: public

BBa_I746350
type: Component
persistent identity: component2055282
display id: component2055282
version: 1
top level: BBa_K274370
definition: BBa_I746350
access: public

BBa_I746360
type: Component
persistent identity: component2055290
display id: component2055290
version: 1
top level: BBa_K274370
definition: BBa_I746360
access: public

BBa_B0012
type: Component
persistent identity: component2055285
display id: component2055285
version: 1
top level: BBa_K274370
definition: BBa_B0012
access: public

BBa_I746350
type: SequenceAnnotation
persistent identity: annotation2055282
display id: annotation2055282
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055282/range2055282/1
component: BBa_I746350

https://synbiohub.org/public/igem/BBa_K274370/annotation2055282/range2055282/1
type: Range
persistent identity: range2055282
display id: range2055282
version: 1
top level: BBa_K274370
direction: direction/reverse
start: 1
end: 237
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2055283
display id: annotation2055283
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055283/range2055283/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K274370/annotation2055283/range2055283/1
type: Range
persistent identity: range2055283
display id: range2055283
version: 1
top level: BBa_K274370
direction: direction/forward
start: 246
end: 325
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2055285
display id: annotation2055285
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055285/range2055285/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K274370/annotation2055285/range2055285/1
type: Range
persistent identity: range2055285
display id: range2055285
version: 1
top level: BBa_K274370
direction: direction/forward
start: 334
end: 374
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2055290
display id: annotation2055290
version: 1
top level: BBa_K274370
location: https://synbiohub.org/public/igem/BBa_K274370/annotation2055290/range2055290/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K274370/annotation2055290/range2055290/1
type: Range
persistent identity: range2055290
display id: range2055290
version: 1
top level: BBa_K274370
direction: direction/reverse
start: 383
end: 473
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_I746350
type: ComponentDefinition
persistent identity: BBa I746350
display id: BBa_I746350
version: 1
was generated by: iGEM to SBOL conversion
description: ogr activator from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The ogr activator taken from P2 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: B0034
sequence annotation: P2 ogr
sequence: https://synbiohub.org/public/igem/BBa_I746350_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943865
display id: annotation1943865
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746350/annotation1943865/range1943865/1
type: Range
persistent identity: range1943865
display id: range1943865
version: 1
top level: BBa_I746350
direction: direction/forward
start: 1
end: 12
orientation: inline

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943867
display id: annotation1943867
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746350/annotation1943867/range1943867/1
type: Range
persistent identity: range1943867
display id: range1943867
version: 1
top level: BBa_I746350
direction: direction/forward
start: 19
end: 237
orientation: inline

P2 ogr
type: SequenceAnnotation
persistent identity: annotation1943866
display id: annotation1943866
version: 1
top level: BBa_I746350
location: https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_I746350/annotation1943866/range1943866/1
type: Range
persistent identity: range1943866
display id: range1943866
version: 1
top level: BBa_I746350
direction: direction/forward
start: 19
end: 19
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_I746360
type: ComponentDefinition
persistent identity: BBa I746360
display id: BBa_I746360
version: 1
was generated by: iGEM to SBOL conversion
description: PF promoter from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The original source is the P2 phage genome. For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations seemed necessary
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PF
sequence: https://synbiohub.org/public/igem/BBa_I746360_sequence/1

PF
type: SequenceAnnotation
persistent identity: annotation1943783
display id: annotation1943783
version: 1
top level: BBa_I746360
location: https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
type: Range
persistent identity: range1943783
display id: range1943783
version: 1
top level: BBa_I746360
direction: direction/forward
start: 1
end: 91
orientation: inline

https://synbiohub.org/public/igem/BBa_K614001_sequence/1
type: Sequence
persistent identity: BBa K614001 sequence
display id: BBa_K614001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagaaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081014_sequence/1
type: Sequence
persistent identity: BBa K081014 sequence
display id: BBa_K081014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K216005_sequence/1
type: Sequence
persistent identity: BBa K216005 sequence
display id: BBa_K216005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K274370_sequence/1
type: Sequence
persistent identity: BBa K274370 sequence
display id: BBa_K274370_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746350_sequence/1
type: Sequence
persistent identity: BBa I746350 sequence
display id: BBa_I746350_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746360_sequence/1
type: Sequence
persistent identity: BBa I746360 sequence
display id: BBa_I746360_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccact
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z