LOCUS       BBa_K614002             2945 bp    DNA     linear       23-Dec-2024
DEFINITION  Nitrate responsive promoter with BamH1 production for safety
ACCESSION   BBa_K614002
VERSION     BBa_K614002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1918
                     /label=BBa_I716462
     stem_loop       1938..1981
                     /label=stem_loop
     misc_feature    1927..2006
                     /label=BBa_B0010
     terminator      1927..2006
                     /label=BBa_B0010
     stem_loop       2022..2041
                     /label=T7 TE
     misc_feature    2048..2048
                     /label=stop
     polyA_site      2042..2055
                     /label=polya
     misc_feature    2015..2055
                     /label=BBa_B0012
     terminator      2015..2055
                     /label=BBa_B0012
     terminator      1927..2055
                     /label=BBa_B0015
     misc_feature    2113..2128
                     /label=NarL-binding region
     misc_feature    2121..2143
                     /label=NsrR-binding region
     misc_feature    2129..2134
                     /label=promoter -35 region
     misc_feature    2152..2157
                     /label=promoter -10 region
     promoter        2064..2163
                     /label=BBa_K216005
     RBS             2172..2186
                     /label=RBS-1\Strong
     RBS             2179..2183
                     /label=RBS
     misc_feature    2172..2186
                     /label=BBa_B0030
     RBS             2172..2186
                     /label=BBa_B0030
     CDS             2193..2867
                     /label=mrfp1
     misc_feature    2874..2898
                     /label=Help:Barcodes
     CDS             2193..2898
                     /label=BBa_E1010
     misc_feature    2907..2945
                     /label=B1006
     stem_loop       2916..2937
                     /label=modified thr terminator
     polyA_site      2938..2945
                     /label=PolyA
     polyA_site      2907..2915
                     /label=PolyA
     terminator      2907..2945
                     /label=BBa_B1006
     misc_feature    2172..2945
                     /label=BBa_K081014
     misc_feature    2064..2945
                     /label=BBa_K614000
ORIGIN
        1 gatctctatg ctactccatc gagccgtcaa ttgtctgatt cgttaccaat tatgacaact
       61 tgacggctac atcattcact ttttcttcac aaccggcacg gaactcgctc gggctggccc
      121 cggtgcattt tttaaatacc cgcgagaaat agagttgatc gtcaaaacca acattgcgac
      181 cgacggtggc gataggcatc cgggtggtgc tcaaaagcag cttcgcctgg ctgatacgtt
      241 ggtcctcgcg ccagcttaag acgctaatcc ctaactgctg gcggaaaaga tgtgacagac
      301 gcgacggcga caagcaaaca tgctgtgcga cgctggcgat atcaaaattg ctgtctgcca
      361 ggtgatcgct gatgtactga caagcctcgc gtacccgatt atccatcggt ggatggagcg
      421 actcgttaat cgcttccatg cgccgcagta acaattgctc aagcagattt atcgccagca
      481 gctccgaata gcgcccttcc ccttgcccgg cgttaatgat ttgcccaaac aggtcgctga
      541 aatgcggctg gtgcgcttca tccgggcgaa agaaccccgt attggcaaat attgacggcc
      601 agttaagcca ttcatgccag taggcgcgcg gacgaaagta aacccactgg tgataccatt
      661 cgcgagcctc cggatgacga ccgtagtgat gaatctctcc tggcgggaac agcaaaatat
      721 cacccggtcg gcaaacaaat tctcgtccct gatttttcac caccccctga ccgcgaatgg
      781 tgagattgag aatataacct ttcattccca gcggtcggtc gataaaaaaa tcgagataac
      841 cgttggcctc aatcggcgtt aaacccgcca ccagatgggc attaaacgag tatcccggca
      901 gcaggggatc attttgcgct tcagccatac ttttcatact cccgccattc agagaagaaa
      961 ccaattgtcc atattgcatc agacattgcc gtcactgcgt cttttactgg ctcttctcgc
     1021 taaccaaacc ggtaaccccg cttattaaaa gcattctgta acaaagcggg accaaagcca
     1081 tgacaaaaac gcgtaacaaa agtgtctata atcacggcag aaaagtccac attgattatt
     1141 tgcacggcgt cacactttgc tatgccatag catttttatc cataagatta gcggatctta
     1201 cctgacgctt tttatcgcaa ctctctactg tttctccata cccggatctg gctaacatag
     1261 ggtggatcta tgggatctga agtagaaaaa gagtttatta ctgatgaagc aaaagaatta
     1321 ctatctaaag ataagttaat tcaacaagca tacaatgaag ttaaaacatc tatttgttca
     1381 cctatttggc cagcaacctc aaaaaccttc acgattaaca acaccgaaaa gaattgtaac
     1441 ggtgtagtac caattaaaga actatgttac accttacttg aagatacata taactggtat
     1501 agagaaaaac cccttgatat acttaaactt gaaaagaaaa aaggtggtcc gattgatgtt
     1561 tataaagagt tcatagaaaa cagtgaactt aaacgtgtag gtatggaatt tgaaacagga
     1621 aatattagtt ctgcccaccg ttcaatgaac aaacttctat taggattaaa acatggcgaa
     1681 attgatttgg ctattatcct tatgcctatt aaacaattgg cctattatct tacagatcgt
     1741 gttaccaatt tcgaggaatt agaaccttat tttgaactta ctgaaggaca accatttatt
     1801 tttattggat ttaatgctga ggcttataat tctaatgtcc ctttaattcc caaaggttct
     1861 gacggtatgt caaaacgctc aattaagaaa tggaaagata aagttgaaaa caaataggta
     1921 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
     1981 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     2041 ctttctgcgt ttatatacta gagttcccat ctataatcct ccctgattct tcgctgatat
     2101 ggtgctaaaa agtaaccaat aaatggtatt taaaatgcaa attatcaggc gtaccctgaa
     2161 acgtactaga gattaaagag gagaaatact agatggcttc ctccgaagac gttatcaaag
     2221 agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg
     2281 aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca
     2341 aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca
     2401 aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag
     2461 gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg
     2521 actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc
     2581 cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta
     2641 tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg
     2701 gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc
     2761 cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca
     2821 tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat
     2881 agtgctagtg tagatcgcta ctagagaaaa aaaaaccccg cccctgacag ggcggggttt
     2941 ttttt
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