##gff-version 3
##sequence-region BBa_K614002 1 2945
BBa_K614002	.	terminator	1927	2055	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K614002	.	terminator	1927	2006	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K614002	.	engineered_region	1927	2006	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K614002	.	stem_loop	1938	1981	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K614002	.	terminator	2015	2055	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K614002	.	stop_codon	2048	2048	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K614002	.	polyA_site	2042	2055	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K614002	.	stem_loop	2022	2041	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K614002	.	engineered_region	2015	2055	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K614002	.	sequence_feature	1	1918	.	+	0	ID=BBa_I716462;Name=BBa_I716462
BBa_K614002	.	engineered_region	2064	2945	.	+	0	ID=BBa_K614000;Name=BBa_K614000
BBa_K614002	.	promoter	2064	2163	.	+	0	ID=BBa_K216005;Name=BBa_K216005;Parent=BBa_K614000
BBa_K614002	.	non_covalent_binding_site	2113	2128	.	+	0	ID=annotation2027072;Name=NarL-binding region;Parent=BBa_K216005
BBa_K614002	.	non_covalent_binding_site	2121	2143	.	+	0	ID=annotation2027073;Name=NsrR-binding region;Parent=BBa_K216005
BBa_K614002	.	non_covalent_binding_site	2129	2134	.	+	0	ID=annotation2027074;Name=promoter -35 region;Parent=BBa_K216005
BBa_K614002	.	non_covalent_binding_site	2152	2157	.	+	0	ID=annotation2027075;Name=promoter -10 region;Parent=BBa_K216005
BBa_K614002	.	engineered_region	2172	2945	.	+	0	ID=BBa_K081014;Name=BBa_K081014;Parent=BBa_K614000
BBa_K614002	.	ribosome_entry_site	2172	2186	.	+	0	ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K081014
BBa_K614002	.	ribosome_entry_site	2179	2183	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K614002	.	engineered_region	2172	2186	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K614002	.	ribosome_entry_site	2172	2186	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K614002	.	CDS	2193	2898	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K081014
BBa_K614002	.	engineered_tag	2874	2898	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K614002	.	CDS	2193	2867	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K614002	.	terminator	2907	2945	.	+	0	ID=BBa_B1006;Name=BBa_B1006;Parent=BBa_K081014
BBa_K614002	.	polyA_site	2907	2915	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K614002	.	polyA_site	2938	2945	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K614002	.	stem_loop	2916	2937	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K614002	.	engineered_region	2907	2945	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
>BBa_K614002
gatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcac
tttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttga
tcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatac
gttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagca
aacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacc
cgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcg
ccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtg
cgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcgga
cgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaaca
gcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgag
aatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgcc
accagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccat
tcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaa
accggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtc
tataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataa
gattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctggctaacatagggtg
gatctatgggatctgaagtagaaaaagagtttattactgatgaagcaaaagaattactatctaaagataagttaattca
acaagcatacaatgaagttaaaacatctatttgttcacctatttggccagcaacctcaaaaaccttcacgattaacaac
accgaaaagaattgtaacggtgtagtaccaattaaagaactatgttacaccttacttgaagatacatataactggtata
gagaaaaaccccttgatatacttaaacttgaaaagaaaaaaggtggtccgattgatgtttataaagagttcatagaaaa
cagtgaacttaaacgtgtaggtatggaatttgaaacaggaaatattagttctgcccaccgttcaatgaacaaacttcta
ttaggattaaaacatggcgaaattgatttggctattatccttatgcctattaaacaattggcctattatcttacagatc
gtgttaccaatttcgaggaattagaaccttattttgaacttactgaaggacaaccatttatttttattggatttaatgc
tgaggcttataattctaatgtccctttaattcccaaaggttctgacggtatgtcaaaacgctcaattaagaaatggaaa
gataaagttgaaaacaaataggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcg
ttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttat
atactagagttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaa
aatgcaaattatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatggcttcctccgaagacgt
tatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggt
gaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggaca
tcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtc
cttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctcc
ctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaa
aaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtct
gaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggt
gcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctg
aaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgccc
ctgacagggcggggtttttttt