BBa_K614002
type: ComponentDefinition
persistent identity: BBa K614002
display id: BBa_K614002
version: 1
was generated by: iGEM to SBOL conversion
description: Nitrate responsive promoter with BamH1 production for safety
created: 2011-10-08T11:00:00Z
modified: 2015-05-08T01:12:54Z
mutable provenance: This part is composed of pre-existing biobrick parts. The BamH1 was submitted by the UC Berkeley 2007 team.
owned by: james
owned by: myers
mutable description: This part contains the PyeaR-RFP cassette with the BamH1 coding region under an Arabinose inducible promoter to trigger the death of the bacterium.
discontinued: false
dominant: false
group u list: _786_
m user id: 0
owner id: 9218
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: None.
star: false
creator: Steven Allen
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_I716462
component: BBa_K614000
component: BBa_B0015
sequence annotation: BBa_I716462
sequence annotation: BBa_B0015
sequence annotation: BBa_K614000
sequence: https://synbiohub.org/public/igem/BBa_K614002_sequence/1

BBa_I716462
type: Component
persistent identity: component2268825
display id: component2268825
version: 1
top level: BBa_K614002
definition: BBa_I716462
access: public

BBa_K614000
type: Component
persistent identity: component2268850
display id: component2268850
version: 1
top level: BBa_K614002
definition: BBa_K614000
access: public

BBa_B0015
type: Component
persistent identity: component2268832
display id: component2268832
version: 1
top level: BBa_K614002
definition: BBa_B0015
access: public

BBa_I716462
type: SequenceAnnotation
persistent identity: annotation2268825
display id: annotation2268825
version: 1
top level: BBa_K614002
location: https://synbiohub.org/public/igem/BBa_K614002/annotation2268825/range2268825/1
component: BBa_I716462

https://synbiohub.org/public/igem/BBa_K614002/annotation2268825/range2268825/1
type: Range
persistent identity: range2268825
display id: range2268825
version: 1
top level: BBa_K614002
direction: direction/reverse
start: 1
end: 1918
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2268832
display id: annotation2268832
version: 1
top level: BBa_K614002
location: https://synbiohub.org/public/igem/BBa_K614002/annotation2268832/range2268832/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K614002/annotation2268832/range2268832/1
type: Range
persistent identity: range2268832
display id: range2268832
version: 1
top level: BBa_K614002
direction: direction/reverse
start: 1927
end: 2055
orientation: inline

BBa_K614000
type: SequenceAnnotation
persistent identity: annotation2268850
display id: annotation2268850
version: 1
top level: BBa_K614002
location: https://synbiohub.org/public/igem/BBa_K614002/annotation2268850/range2268850/1
component: BBa_K614000

https://synbiohub.org/public/igem/BBa_K614002/annotation2268850/range2268850/1
type: Range
persistent identity: range2268850
display id: range2268850
version: 1
top level: BBa_K614002
direction: direction/reverse
start: 2064
end: 2945
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I716462
type: ComponentDefinition
persistent identity: BBa I716462
display id: BBa_I716462
version: 1
was generated by: iGEM to SBOL conversion
description: {AraC-Pbad}{rbs1-A}{ATG}{BamHI no ATG}
created: 2007-10-22T11:00:00Z
modified: 2015-08-31T04:07:52Z
mutable provenance: I716450 + I716210
owned by: james
owned by: myers
mutable description: {AraC-Pbad}{rbs1-A}{ATG}{BamHI no ATG}
discontinued: false
dominant: false
group u list: _110_
m user id: 0
owner id: 933
owning group id: 52
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This part is in biobricks 2.0 with BamHI and BglII sites instead of PstI and SpeI sites
star: true
creator: Samantha Liang
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_I716462_sequence/1

BBa_K614000
type: ComponentDefinition
persistent identity: BBa K614000
display id: BBa_K614000
version: 1
was generated by: iGEM to SBOL conversion
description: PyeaR promoter with RFP reporter
created: 2011-09-22T11:00:00Z
modified: 2015-05-08T01:12:54Z
mutable provenance: All parts of this construct are from previously submitted biobricks.
owned by: james
owned by: myers
mutable description: PyeaR is a nitrate responsive promoter driving a red fluorescent protein reporter.
discontinued: false
dominant: false
experience: experience/Works
group u list: _786_
m user id: 0
owner id: 4261
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None.
star: false
creator: Anne Byford
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K081014
component: BBa_K216005
sequence annotation: BBa_K216005
sequence annotation: BBa_K081014
sequence: https://synbiohub.org/public/igem/BBa_K614000_sequence/1

BBa_K081014
type: Component
persistent identity: component2250717
display id: component2250717
version: 1
top level: BBa_K614000
definition: BBa_K081014
access: public

BBa_K216005
type: Component
persistent identity: component2250705
display id: component2250705
version: 1
top level: BBa_K614000
definition: BBa_K216005
access: public

BBa_K216005
type: SequenceAnnotation
persistent identity: annotation2250705
display id: annotation2250705
version: 1
top level: BBa_K614000
location: https://synbiohub.org/public/igem/BBa_K614000/annotation2250705/range2250705/1
component: BBa_K216005

https://synbiohub.org/public/igem/BBa_K614000/annotation2250705/range2250705/1
type: Range
persistent identity: range2250705
display id: range2250705
version: 1
top level: BBa_K614000
direction: direction/reverse
start: 1
end: 100
orientation: inline

BBa_K081014
type: SequenceAnnotation
persistent identity: annotation2250717
display id: annotation2250717
version: 1
top level: BBa_K614000
location: https://synbiohub.org/public/igem/BBa_K614000/annotation2250717/range2250717/1
component: BBa_K081014

https://synbiohub.org/public/igem/BBa_K614000/annotation2250717/range2250717/1
type: Range
persistent identity: range2250717
display id: range2250717
version: 1
top level: BBa_K614000
direction: direction/reverse
start: 109
end: 882
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K081014
type: ComponentDefinition
persistent identity: BBa K081014
display id: BBa_K081014
version: 1
was generated by: iGEM to SBOL conversion
description: RFP protein generator - PoPS->RFP
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: RBS: RFP: T:
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PoPS in -> RFP out Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
discontinued: false
dominant: true
experience: experience/Works
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0030
component: BBa_B1006
component: BBa_E1010
sequence annotation: BBa_B0030
sequence annotation: BBa_E1010
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081014_sequence/1

BBa_B0030
type: Component
persistent identity: component2246146
display id: component2246146
version: 1
top level: BBa_K081014
definition: BBa_B0030
access: public

BBa_B1006
type: Component
persistent identity: component2246155
display id: component2246155
version: 1
top level: BBa_K081014
definition: BBa_B1006
access: public

BBa_E1010
type: Component
persistent identity: component2246150
display id: component2246150
version: 1
top level: BBa_K081014
definition: mRFP1
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246146
display id: annotation2246146
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081014/annotation2246146/range2246146/1
type: Range
persistent identity: range2246146
display id: range2246146
version: 1
top level: BBa_K081014
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2246150
display id: annotation2246150
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K081014/annotation2246150/range2246150/1
type: Range
persistent identity: range2246150
display id: range2246150
version: 1
top level: BBa_K081014
direction: direction/reverse
start: 22
end: 727
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246155
display id: annotation2246155
version: 1
top level: BBa_K081014
location: https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081014/annotation2246155/range2246155/1
type: Range
persistent identity: range2246155
display id: range2246155
version: 1
top level: BBa_K081014
direction: direction/reverse
start: 736
end: 774
orientation: inline

BBa_K216005
type: ComponentDefinition
persistent identity: BBa K216005
display id: BBa_K216005
version: 1
was generated by: iGEM to SBOL conversion
description: PyeaR promoter, responsive to nitrate, nitrite and nitric oxide
created: 2009-09-24T11:00:00Z
modified: 2015-05-08T01:11:30Z
mutable provenance: BioBricked from E. coli K-12 genomic DNA by the Edinburgh 2009 iGEM team.
owned by: james
owned by: myers
mutable description: PyeaR promoter. This is the promoter of the Escherichia coli yeaR/yoaG operon (see Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in ''Escherichia coli'' K-12. J. Bacteriol. 189, 7539-7548). Unlike other E. coli promoters responding to nitrate and nitrite, this promoter is not repressed under aerobic conditions.
discontinued: false
dominant: false
experience: experience/Works
group u list: _317_
m user id: 0
owner id: 837
owning group id: 163
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No special considerations.
star: false
creator: Edinburgh iGEM 2009
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: NarL-binding region
sequence annotation: NsrR-binding region
sequence annotation: promoter -35 region
sequence annotation: promoter -10 region
sequence: https://synbiohub.org/public/igem/BBa_K216005_sequence/1

NarL-binding region
type: SequenceAnnotation
persistent identity: annotation2027072
display id: annotation2027072
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027072/range2027072/1
type: Range
persistent identity: range2027072
display id: range2027072
version: 1
top level: BBa_K216005
direction: direction/forward
start: 50
end: 65
orientation: inline

NsrR-binding region
type: SequenceAnnotation
persistent identity: annotation2027073
display id: annotation2027073
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027073/range2027073/1
type: Range
persistent identity: range2027073
display id: range2027073
version: 1
top level: BBa_K216005
direction: direction/forward
start: 58
end: 80
orientation: inline

promoter -35 region
type: SequenceAnnotation
persistent identity: annotation2027074
display id: annotation2027074
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027074/range2027074/1
type: Range
persistent identity: range2027074
display id: range2027074
version: 1
top level: BBa_K216005
direction: direction/forward
start: 66
end: 71
orientation: inline

promoter -10 region
type: SequenceAnnotation
persistent identity: annotation2027075
display id: annotation2027075
version: 1
top level: BBa_K216005
location: https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K216005/annotation2027075/range2027075/1
type: Range
persistent identity: range2027075
display id: range2027075
version: 1
top level: BBa_K216005
direction: direction/forward
start: 89
end: 94
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

https://synbiohub.org/public/igem/BBa_K614002_sequence/1
type: Sequence
persistent identity: BBa K614002 sequence
display id: BBa_K614002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctggctaacatagggtggatctatgggatctgaagtagaaaaagagtttattactgatgaagcaaaagaattactatctaaagataagttaattcaacaagcatacaatgaagttaaaacatctatttgttcacctatttggccagcaacctcaaaaaccttcacgattaacaacaccgaaaagaattgtaacggtgtagtaccaattaaagaactatgttacaccttacttgaagatacatataactggtatagagaaaaaccccttgatatacttaaacttgaaaagaaaaaaggtggtccgattgatgtttataaagagttcatagaaaacagtgaacttaaacgtgtaggtatggaatttgaaacaggaaatattagttctgcccaccgttcaatgaacaaacttctattaggattaaaacatggcgaaattgatttggctattatccttatgcctattaaacaattggcctattatcttacagatcgtgttaccaatttcgaggaattagaaccttattttgaacttactgaaggacaaccatttatttttattggatttaatgctgaggcttataattctaatgtccctttaattcccaaaggttctgacggtatgtcaaaacgctcaattaagaaatggaaagataaagttgaaaacaaataggtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I716462_sequence/1
type: Sequence
persistent identity: BBa I716462 sequence
display id: BBa_I716462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gatctctatgctactccatcgagccgtcaattgtctgattcgttaccaattatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcttacctgacgctttttatcgcaactctctactgtttctccatacccggatctggctaacatagggtggatctatgggatctgaagtagaaaaagagtttattactgatgaagcaaaagaattactatctaaagataagttaattcaacaagcatacaatgaagttaaaacatctatttgttcacctatttggccagcaacctcaaaaaccttcacgattaacaacaccgaaaagaattgtaacggtgtagtaccaattaaagaactatgttacaccttacttgaagatacatataactggtatagagaaaaaccccttgatatacttaaacttgaaaagaaaaaaggtggtccgattgatgtttataaagagttcatagaaaacagtgaacttaaacgtgtaggtatggaatttgaaacaggaaatattagttctgcccaccgttcaatgaacaaacttctattaggattaaaacatggcgaaattgatttggctattatccttatgcctattaaacaattggcctattatcttacagatcgtgttaccaatttcgaggaattagaaccttattttgaacttactgaaggacaaccatttatttttattggatttaatgctgaggcttataattctaatgtccctttaattcccaaaggttctgacggtatgtcaaaacgctcaattaagaaatggaaagataaagttgaaaacaaatagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K614000_sequence/1
type: Sequence
persistent identity: BBa K614000 sequence
display id: BBa_K614000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacgtactagagattaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081014_sequence/1
type: Sequence
persistent identity: BBa K081014 sequence
display id: BBa_K081014_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K216005_sequence/1
type: Sequence
persistent identity: BBa K216005 sequence
display id: BBa_K216005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttcccatctataatcctccctgattcttcgctgatatggtgctaaaaagtaaccaataaatggtatttaaaatgcaaattatcaggcgtaccctgaaacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z