LOCUS       BBa_K615000             2593 bp    DNA     linear       22-Dec-2024
DEFINITION  E. coli strain for His3-URA3 one-hybrid selection system
ACCESSION   BBa_K615000
VERSION     BBa_K615000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..950
                     /label=Kanamycin resistance cassette
     promoter        950..1080
                     /label=Target and promoter region
     misc_feature    978..986
                     /label=Zif268 binding site
     CDS             1080..1742
                     /label=His3
     CDS             1790..2593
                     /label=URA3
ORIGIN
        1 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
       61 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      121 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      181 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      241 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
      301 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      361 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
      421 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      481 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      541 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      601 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
      661 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
      721 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
      781 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
      841 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
      901 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc cctgcaggtg
      961 tcgaagatct tcgacatgcg tgggcggatt acattaggca ccccgggctt tacactttat
     1021 gcttccggct cgtatgttgt gtcgaccgag cggataacaa tttcacacag gaaacagcta
     1081 tgacagagca gaaagcccta gtaaagcgta ttacaaatga aaccaagatt cagattgcga
     1141 tctctttaaa gggtggtccc ctagcgatag agcactcgat cttcccagaa aaagaggcag
     1201 aagcagtagc agaacaggcc acacaatcgc aagtgattaa cgtccacaca ggtatagggt
     1261 ttctggacca catgatacat gctctggcca agcattccgg ctggtcgcta atcgttgagt
     1321 gcattggtga cttacacata gacgaccatc acaccactga agactgcggg attgctctcg
     1381 gtcaggcttt taaagaggcc ctaggggccg tgcgtggagt aaaaaggttt ggatcaggat
     1441 ttgcgccttt ggatgaggca ctttccagag cggtggtaga tctttcgaac aggccatacg
     1501 cagttgtcga acttggtttg caaagggaga aagtaggaga tctctcttgc gagatgatcc
     1561 cgcattttct tgagagcttt gcagaggcca gcagaattac cctccacgtt gattgtctgc
     1621 gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa ggctcttgcg gttgccataa
     1681 gagaagccac ctcgcccaat ggaaccaacg atgttccctc caccaaaggt gttcttatgt
     1741 agtgacaccg attatttaaa gctgcaggga tcccacacag gaaacagcta tgtcgaaagc
     1801 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
     1861 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
     1921 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
     1981 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
     2041 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
     2101 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
     2161 ggtgggccca ggtattgtta gcggtttgaa gcaggcggcg gaagaagtaa caaaggaacc
     2221 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctagcta ctggagaata
     2281 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
     2341 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     2401 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     2461 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     2521 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     2581 ccagcaaaac taa
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