LOCUS       BBa_K615003             1083 bp    DNA     linear       22-Nov-2024
DEFINITION  E. coli strain for TolC one-hybrid selection system
ACCESSION   BBa_K615003
VERSION     BBa_K615003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..950
                     /label=kanamycin cassette
     promoter        951..1046
                     /label=target and promoter region
     misc_feature    978..986
                     /label=Zif268 binding site
ORIGIN
        1 tcgaacccca gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct
       61 gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa
      121 gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca
      181 gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc
      241 aggcatcgcc atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg
      301 cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa
      361 gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg
      421 ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt
      481 tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca
      541 gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg
      601 tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt
      661 cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag
      721 agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg
      781 gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt
      841 gatcagatct tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta
      901 ctttgcaggg cttcccaacc ttaccagagg gcgccccagc tggcaattcc cctgcaggtg
      961 tcgaagatct tcgacatgcg tgggcggatt acattaggca ccccgggctt tacactttat
     1021 gcttccggct cgtatgttgt gtcgaccgag cggataacaa tttcacacag gaaacagcta
     1081 tga
//