BBa_K615003 1 BBa_K615003 E. coli strain for TolC one-hybrid selection system 2011-09-25T11:00:00Z 2015-05-08T01:12:54Z Based off EcNR2 selection strain (see Wang et al, 2009) This strain is designed to test zinc finger binding by tying the binding event to cell survival, specifically the expression of TolC, a membrane pump that removes toxins from the cell. false false _787_ 0 10031 9 It's complicated true rpoZ knocked out using lambda red Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC false Naomi Genuth annotation2142671 1 kanamycin cassette range2142671 1 1 950 annotation2142672 1 target and promoter region range2142672 1 951 1046 annotation2142673 1 Zif268 binding site range2142673 1 978 986 BBa_K615003_sequence 1 tcgaaccccagagtcccgctcagaagaactcgtcaagaaggcgatagaaggcgatgcgctgcgaatcgggagcggcgataccgtaaagcacgaggaagcggtcagcccattcgccgccaagctcttcagcaatatcacgggtagccaacgctatgtcctgatagcggtccgccacacccagccggccacagtcgatgaatccagaaaagcggccattttccaccatgatattcggcaagcaggcatcgccatgggtcacgacgagatcctcgccgtcgggcatgcgcgccttgagcctggcgaacagttcggctggcgcgagcccctgatgctcttcgtccagatcatcctgatcgacaagaccggcttccatccgagtacgtgctcgctcgatgcgatgtttcgcttggtggtcgaatgggcaggtagccggatcaagcgtatgcagccgccgcattgcatcagccatgatggatactttctcggcaggagcaaggtgagatgacaggagatcctgccccggcacttcgcccaatagcagccagtcccttcccgcttcagtgacaacgtcgagcacagctgcgcaaggaacgcccgtcgtggccagccacgatagccgcgctgcctcgtcctgcagttcattcagggcaccggacaggtcggtcttgacaaaaagaaccgggcgcccctgcgctgacagccggaacacggcggcatcagagcagccgattgtctgttgtgcccagtcatagccgaatagcctctccacccaagcggccggagaacctgcgtgcaatccatcttgttcaatcatgcgaaacgatcctcatcctgtctcttgatcagatcttgatcccctgcgccatcagatccttggcggcaagaaagccatccagtttactttgcagggcttcccaaccttaccagagggcgccccagctggcaattcccctgcaggtgtcgaagatcttcgacatgcgtgggcggattacattaggcaccccgggctttacactttatgcttccggctcgtatgttgtgtcgaccgagcggataacaatttcacacaggaaacagctatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z