LOCUS       BBa_K615004             1643 bp    DNA     linear       23-Dec-2024
DEFINITION  Zif268 binding site-His3-URA3 construct for one-hybrid selection
ACCESSION   BBa_K615004
VERSION     BBa_K615004.1  
FEATURES             Location/Qualifiers
     promoter        1..96
                     /label=target and weak promoter
     misc_feature    28..36
                     /label=Zif268 binding site
     CDS             130..792
                     /label=His3
     CDS             840..1640
                     /label=URA3
ORIGIN
        1 cttgcaggtg tcgaagatct tcgacatgcg tgggcggatt acattaggca ccccgggctt
       61 tacactttat gcttccggct cgtatgttgt gtcgaccgag cggataacaa tttcacacag
      121 gaaacagcta tgacagagca gaaagcccta gtaaagcgta ttacaaatga aaccaagatt
      181 cagattgcga tctctttaaa gggtggtccc ctagcgatag agcactcgat cttcccagaa
      241 aaagaggcag aagcagtagc agaacaggcc acacaatcgc aagtgattaa cgtccacaca
      301 ggtatagggt ttctggacca catgatacat gctctggcca agcattccgg ctggtcgcta
      361 atcgttgagt gcattggtga cttacacata gacgaccatc acaccactga agactgcggg
      421 attgctctcg gtcaggcttt taaagaggcc ctaggggccg tgcgtggagt aaaaaggttt
      481 ggatcaggat ttgcgccttt ggatgaggca ctttccagag cggtggtaga tctttcgaac
      541 aggccatacg cagttgtcga acttggtttg caaagggaga aagtaggaga tctctcttgc
      601 gagatgatcc cgcattttct tgagagcttt gcagaggcca gcagaattac cctccacgtt
      661 gattgtctgc gaggcaagaa tgatcatcac cgtagtgaga gtgcgttcaa ggctcttgcg
      721 gttgccataa gagaagccac ctcgcccaat ggaaccaacg atgttccctc caccaaaggt
      781 gttcttatgt agtgacaccg attatttaaa gttgcaggga tcccacacag gaaacagcta
      841 tgtcgaaagc tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc
      901 tatttaatat catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca
      961 ccaaggaatt actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac
     1021 atgtggatat cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat
     1081 ccgccaagta caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag
     1141 tcaaattgca gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg
     1201 cacacggtgt ggtgggccca ggtattgtta gcggtttgaa gcaggcggcg gaagaagtaa
     1261 caaaggaacc tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctagcta
     1321 ctggagaata tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg
     1381 gctttattgc tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga
     1441 cacccggtgt gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg
     1501 atgatgtggt ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg
     1561 gaagggatgc taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga
     1621 gaagatgcgg ccagcaaaac taa
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