BBa_K615004 1 BBa_K615004 Zif268 binding site-His3-URA3 construct for one-hybrid selection 2011-09-25T11:00:00Z 2015-05-08T01:12:54Z The sequence was synthesized. This is one of the two components necessary for a metabolic His3-URA3 selection system (the other being a zinc finger fused to the omega subunit of RNA polymerase). false false _787_ 0 10031 9 It's complicated true Zif268 binding site in the promoter region to initiate expression of His3 and URA3 only when a zinc finger is bound properly. false Naomi Genuth annotation2144671 1 target and weak promoter range2144671 1 1 96 annotation2144674 1 URA3 range2144674 1 840 1640 annotation2144672 1 Zif268 binding site range2144672 1 28 36 annotation2144673 1 His3 range2144673 1 130 792 BBa_K615004_sequence 1 cttgcaggtgtcgaagatcttcgacatgcgtgggcggattacattaggcaccccgggctttacactttatgcttccggctcgtatgttgtgtcgaccgagcggataacaatttcacacaggaaacagctatgacagagcagaaagccctagtaaagcgtattacaaatgaaaccaagattcagattgcgatctctttaaagggtggtcccctagcgatagagcactcgatcttcccagaaaaagaggcagaagcagtagcagaacaggccacacaatcgcaagtgattaacgtccacacaggtatagggtttctggaccacatgatacatgctctggccaagcattccggctggtcgctaatcgttgagtgcattggtgacttacacatagacgaccatcacaccactgaagactgcgggattgctctcggtcaggcttttaaagaggccctaggggccgtgcgtggagtaaaaaggtttggatcaggatttgcgcctttggatgaggcactttccagagcggtggtagatctttcgaacaggccatacgcagttgtcgaacttggtttgcaaagggagaaagtaggagatctctcttgcgagatgatcccgcattttcttgagagctttgcagaggccagcagaattaccctccacgttgattgtctgcgaggcaagaatgatcatcaccgtagtgagagtgcgttcaaggctcttgcggttgccataagagaagccacctcgcccaatggaaccaacgatgttccctccaccaaaggtgttcttatgtagtgacaccgattatttaaagttgcagggatcccacacaggaaacagctatgtcgaaagctacatataaggaacgtgctgctactcatcctagtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaacttgtgtgcttcattggatgttcgtaccaccaaggaattactggagttagttgaagcattaggtcccaaaatttgtttactaaaaacacatgtggatatcttgactgatttttccatggagggcacagttaagccgctaaaggcattatccgccaagtacaattttttactcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacagaatagcagaatgggcagacattacgaatgcacacggtgtggtgggcccaggtattgttagcggtttgaagcaggcggcggaagaagtaacaaaggaacctagaggccttttgatgttagcagaattgtcatgcaagggctccctagctactggagaatatactaagggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaagagacatgggtggaagagatgaaggttacgattggttgattatgacacccggtgtgggtttagatgacaagggagacgcattgggtcaacagtatagaaccgtggatgatgtggtctctacaggatctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggtagagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaactaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z