LOCUS       BBa_K617000             2440 bp    DNA     linear       22-Dec-2024
DEFINITION  Biobrick Compatible Lambda Chromosomal Insertion Plasmid
ACCESSION   BBa_K617000
VERSION     BBa_K617000.1  
FEATURES             Location/Qualifiers
     misc_feature    1..270
                     /label=tL3 terminator
     misc_feature    276..671
                     /label=Lambda attP P'OP
     misc_feature    679..1070
                     /label=R6K Origin of Replication
     CDS             1157..1951
                     /label=KanR
     misc_feature    2116..2375
                     /label=rgnB terminator
     misc_feature    2398..2440
                     /label=Biobrick Site Prefix -> Suffix
ORIGIN
        1 aatggcgatg acgcatcctc acgataatat ccgggtaggc gcaatcactt tcgtctctac
       61 tccgttacaa agcgaggctg ggtatttccc ggcctttctg ttatccgaaa tccactgaaa
      121 gcacagcggc tggctgagga gataaataat aaacgagggg ctgtatgcac aaagcatctt
      181 ctgttgagtt aagaacgagt atcgagatgg cacatagcct tgctcaaatt ggaatcaggt
      241 ttgtgccaat accagtagaa acagacgaag aagctagctt tgcactggat tgcgaggctt
      301 tgtgcttctc tggagtgcga caggtttgat gacaaaaaat tagcgcaaga agacaaaaat
      361 caccttgcgc taatgctctg ttacaggtca ctaataccat ctaagtagtt gattcatagt
      421 gactgcatat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc aaaatctaat
      481 ttaatatatt gatatttata tcattttacg tttctcgttc agctttttta tactaagttg
      541 gcattataaa aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
      601 ataaaatcat tatttgattt caattttgtc ccactccctg cctctgtcat cacgatactg
      661 tgatgccatg gctaattccc atgtcagccg ttaagtgttc ctgtgtcact caaaattgct
      721 ttgagaggct ctaagggctt ctcagtgcgt tacatccctg gcttgttgtc cacaaccgtt
      781 aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa cttaaaacct
      841 tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg agagcttagt
      901 acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta gccatgaggg
      961 tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt acgtgaaaca
     1021 tgagagctta gtacgtacta tcaacaggtt gaactgctga tcttcagatc ctctacgccg
     1081 gacgcatcgt ggccggatct tgcggccgca aaaattaaaa atgaagtttt gacggtatcg
     1141 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     1201 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     1261 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     1321 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     1381 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat ccgcgccttg agcctggcga
     1441 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     1501 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     1561 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     1621 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     1681 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     1741 ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg gacaggtcgg
     1801 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     1861 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     1921 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     1981 ccactagatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     2041 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     2101 gcatcgatgg cccccgatgg tagtgtgggg tctccccatg cgagagtagg gaactgccag
     2161 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     2221 gtcggtgaac gctctcctga gtaggacaaa tccgccggga gcggatttga acgttgcgaa
     2281 gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc atcaaattaa
     2341 gcagaaggcc atcctgacgg atggcctttt tgcgtggcca gtgccaagct tgcatgcgaa
     2401 ttcgcggccg cttctagagt actagtagcg gccgctgcag
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